Pei Huimin, Zhao Mengyuan, Wang Hongyue, Zhang Xindi, Shi Caihong, Zhang Xiangrong
Faculty of Functional Food and Wine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
Food Processing Institute, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin 150086, China.
J Food Drug Anal. 2025 Jun 13;33(2):106-118. doi: 10.38212/2224-6614.3541.
Jiao Sanxian (JSX) consists of three traditional Chinese medicine including charred hawthorn, malt and Liu Shen Qu, they are also from Chinese medicine homologous food. It is commonly used to relieve functional dyspepsia (FD). The present study aims to explore the mechanism of JSX (granule), with the addition of prebiotics for the treatment of FD using mice model. The network pharmacology and molecular docking were used to forecast therapeutic targets for JSX. Based on the protein-protein interaction (PPI) results, thirteen targets exhibited strong interactions with each other. The results of network pharmacology and molecular docking revealed therapeutic activity of JSX in FD regulated by its anti-inflammatory effect, improvement in gastrointestinal function and physiological activity in components-targets-pathways-disease. The pharmacological effects of JSX were further verified by rRNA gene sequencing, enzyme-linked immunosorbent assay (ELISA) and animal study. The in vivo results showed JSX, in combination with prebiotics, could mitigate FD symptoms. ELISA study showed that glyceraldehyde-3-phosphate dehydrogenase (GAPDH), AKT1 and tumor necrosis factor (TNF)-α played the roles in reducing the inflammatory response by downregulating inflammation content. The above results were coincided with the network pharmacology prediction. By 16S rRNA, the results showed JSX may alleviate the FD symptom by increasing the diversity of the intestinal flora, adjusting the dominant prebiotic. In conclusion, JSX can alleviate functional dyspepsia in mice. The mechanisms were related to regulating the secretion of inflammatory response, significantly improving the benefits of intestinal flora and ameliorating multi-metabolic pathways.
焦三仙（JSX）由三种中药组成，包括焦山楂、麦芽和六神曲，它们也是药食同源的食物。它常用于缓解功能性消化不良（FD）。本研究旨在利用小鼠模型探索添加益生元的焦三仙（颗粒剂）治疗FD的机制。采用网络药理学和分子对接技术预测焦三仙的治疗靶点。基于蛋白质-蛋白质相互作用（PPI）结果，有13个靶点相互之间表现出强烈的相互作用。网络药理学和分子对接结果揭示了焦三仙在FD中的治疗活性，其通过抗炎作用、改善胃肠功能以及在成分-靶点-通路-疾病中的生理活性来调节。通过rRNA基因测序、酶联免疫吸附测定（ELISA）和动物研究进一步验证了焦三仙的药理作用。体内实验结果表明，焦三仙与益生元联合使用可减轻FD症状。ELISA研究表明，甘油醛-3-磷酸脱氢酶（GAPDH）、AKT1和肿瘤坏死因子（TNF）-α通过下调炎症含量在减轻炎症反应中发挥作用。上述结果与网络药理学预测结果相符。通过16S rRNA检测，结果表明焦三仙可能通过增加肠道菌群多样性、调节优势益生元来缓解FD症状。总之，焦三仙可缓解小鼠功能性消化不良。其机制与调节炎症反应分泌、显著改善肠道菌群益处以及改善多代谢途径有关。
