Schena Nicholas C, Baker Kassandra M, Stark Anna A, Thomas Derek P, Cleary Ian A
Department of Biomedical Sciences, Grand Valley State University, One Campus Drive, Allendale, MI, 49401, USA.
College of Applied Health Sciences, University of Illinois Chicago, Chicago, IL, USA.
Folia Microbiol (Praha). 2025 Jul 1. doi: 10.1007/s12223-025-01288-y.
The fungal pathogen Candida albicans produces numerous surface proteins involved in adhesion. Many of these adhesins are specific to different morphological forms. Strains that are unable to form hyphae are generally impaired in their ability to adhere to surfaces and to form biofilms. To better understand the function of one of these adhesins, Als3p, in the context of yeast-locked strains, we expressed ALS3 in strains with altered expression of one of two genes that are part of a regulatory circuit, BRG1 and NRG1. The mutant BRG1 and NRG1 strains we examined were phenotypically similar. We observed that ALS3 expression improved the adhesion of both strains to glass but that only one had altered biofilm forming ability, which was decreased.
真菌病原体白色念珠菌产生许多参与黏附的表面蛋白。其中许多黏附素对不同的形态形式具有特异性。无法形成菌丝的菌株通常在黏附于表面和形成生物膜的能力方面受损。为了更好地了解其中一种黏附素Als3p在酵母锁定菌株背景下的功能，我们在参与调控回路的两个基因之一BRG1和NRG1表达发生改变的菌株中表达了ALS3。我们检测的BRG1和NRG1突变菌株在表型上相似。我们观察到ALS3的表达提高了这两种菌株对玻璃的黏附能力，但只有一种菌株的生物膜形成能力发生了改变，且有所降低。
