Ilijin Larisa, Popović Dušanka, Živković Milica, Todorović Dajana, Mrdaković Marija, Vlahović Milena, Perić-Mataruga Vesna
Department of Insect Physiology and Biochemistry, Institute for Biological Research Siniša Stanković, National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blv. 142, 11060, Belgrade, Serbia.
Department of Ecology, Institute for Biological Research Siniša Stanković, National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blv. 142, 11060, Belgrade, Serbia.
Sci Rep. 2025 Jul 1;15(1):21203. doi: 10.1038/s41598-025-04805-8.
This study aimed to analyze the long-term impact of co-fed plastic diet on the bacterial community of self-sustaining laboratory populations of T. molitor fed with wheat bran with added polystyrene (PS), and low density polyethylene (LDPE) over a three year period. The most abundant phyla for all three populations were Firmicutes, Bacteroidota and Proteobacteria. PS group microbiota is similar to C group, pointing to a common bacterial species capable for degrading lignocellulose and PS, while consumption of LDPE caused a significant decrease of Bacteroidota and Actinobacteriota compared to both C and PS group, and Campylobacterota compared to PS group. A predictive metabolomics analysis recognized dTDP-L-rhamnose biosynthesis I in PS group as one of five unique pathways, while other five distinctive pathways, like peptidoglycan maturation, were linked to LDPE group. Further studies are needed to determine the plastic degrading properties of the detected bacteria. The results highlight T. molitor's versatility in biotechnological applications.
本研究旨在分析在三年时间里，共同投喂含塑料饮食对以添加聚苯乙烯（PS）和低密度聚乙烯（LDPE）的麦麸为食的黄粉虫自维持实验室种群细菌群落的长期影响。所有三个种群中最丰富的门是厚壁菌门、拟杆菌门和变形菌门。PS组的微生物群与C组相似，表明存在能够降解木质纤维素和PS的常见细菌物种，而与C组和PS组相比，LDPE的消耗导致拟杆菌门和放线菌门显著减少，与PS组相比弯曲杆菌门减少。预测性代谢组学分析将PS组中的dTDP-L-鼠李糖生物合成I识别为五条独特途径之一，而其他五条独特途径，如肽聚糖成熟，与LDPE组相关。需要进一步研究来确定检测到的细菌的塑料降解特性。结果突出了黄粉虫在生物技术应用中的多功能性。
