Doherty P C, Bartke A, Smith M S
Physiol Behav. 1985 Jul;35(1):99-104. doi: 10.1016/0031-9384(85)90178-7.
To determine if alterations in the availability of the active metabolites of testosterone (T) are involved in the inhibition of sexual activity in hyperprolactinemic animals, the effects of four ectopic pituitary grafts on copulatory behavior were examined in castrated male rats given subcutaneous implants of T or estradiol-17 beta (E2) plus 5 alpha-dihydrotestosterone (DHT). Two weeks after implantation of the steroid-filled capsules, half the animals of each group were given pituitary grafts and the remainder were sham-operated. Tests of copulatory behavior were performed prior to, and one, two, and three months following pituitary transplantation. Pituitary grafting caused significant inhibition of copulatory behavior in both T and E2 + DHT treated animals. PRL levels were significantly higher in E2 + DHT treated grafted males than in T treated grafted animals (2000 +/- 140 vs. 395 +/- 26 ng/ml), but did not differ between the corresponding control groups (61 +/- 8 vs. 73 +/- 6 ng/ml). The results of these experiments preclude the possible involvement of alterations in steroid secretion by the testes or modifications of the conversion of T to its active metabolites in the effects of hyperprolactinemia on copulatory behavior.
为了确定睾酮（T）活性代谢产物的可利用性改变是否参与了高催乳素血症动物的性活动抑制，在皮下植入T或雌二醇-17β（E2）加5α-双氢睾酮（DHT）的去势雄性大鼠中，研究了四个异位垂体移植对交配行为的影响。在植入类固醇填充胶囊两周后，每组动物中的一半接受垂体移植，其余动物进行假手术。在垂体移植前、移植后1个月、2个月和3个月进行交配行为测试。垂体移植对接受T和E2+DHT治疗的动物的交配行为均有显著抑制作用。接受E2+DHT治疗的移植雄性大鼠的催乳素水平显著高于接受T治疗的移植动物（2000±140对395±26 ng/ml），但相应对照组之间无差异（61±8对73±6 ng/ml）。这些实验结果排除了高催乳素血症对交配行为的影响中，睾丸类固醇分泌改变或T向其活性代谢产物转化修饰的可能性。
