Shen Wensheng, Liu Xiangrong, Mu Xinkai, Zhou Anning, Tan Kaili, Nussipov Damir, Tastambek Kuanysh
College of Chemistry and Chemical Engineering, Xi'an University of Science and Technology, Xi'an, 710054, China.
State Key Laboratory of Coal and CBM Co-mining, Jincheng, 048006, China.
Sci Rep. 2025 Jul 1;15(1):21984. doi: 10.1038/s41598-025-08899-y.
Sequential degradation using two bacteria with complementary metabolic capabilities offers a promising strategy for enhancing coal biodegradation efficiency. In this work, the biodegradation processes of the Baode tar-rich coal in Ordos Basin by two sequential treatment modes of N. mangyaensis and Ochrobactrum sp. were investigated. In mode 1, the coal was degraded firstly by N. mangyaensis, and after the degradation process was completed, the separated residual coal was secondly degraded by Ochrobactrum sp.. In mode 2, the stages were the opposite of mode 1. The biodegradation rates of tar-rich coal for mode 1, mode 2, N. mangyaensis and Ochrobactrum sp. were 55.7, 50.1, 43.2, and 29.8%, respectively, indicating that sequential degradation significantly enhanced biodegradation efficiency. Analysis of the solid and liquid products revealed that this difference was due to the distinct degradation characteristics of the bacteria: N. mangyaensis preferentially degraded hydrogen-rich structures, while Ochrobactrum sp. targeted aromatic compounds. The superior performance of mode 1 was attributed to N. mangyaensis initially degrading the hydrogen-rich structures in coal, depolymerizing macromolecules into smaller aromatic compounds and creating more accessible substrates for Ochrobactrum sp., thereby improving the overall biodegradation process. Conversely, in mode 2, Ochrobactrum sp. primarily degraded polycyclic aromatic hydrocarbons in the tar-rich coal during the first stage, resulting in fewer degradable intermediates for the second stage and reducing the overall efficiency of the degradation process. The applicability of this sequential degradation strategy to other types of coal or organic pollutants also warrants exploration, potentially broadening its industrial and environmental relevance.
利用两种具有互补代谢能力的细菌进行顺序降解，为提高煤炭生物降解效率提供了一种很有前景的策略。在这项工作中，研究了曼氏硝化杆菌和慢生根瘤菌的两种顺序处理模式对鄂尔多斯盆地保德富焦油煤的生物降解过程。在模式1中，煤首先由曼氏硝化杆菌降解，降解过程完成后，分离出的残余煤再由慢生根瘤菌降解。在模式2中，阶段与模式1相反。模式1、模式2、曼氏硝化杆菌和慢生根瘤菌对富焦油煤的生物降解率分别为55.7%、50.1%、43.2%和29.8%，表明顺序降解显著提高了生物降解效率。对固体和液体产物的分析表明，这种差异是由于细菌不同的降解特性所致：曼氏硝化杆菌优先降解富氢结构，而慢生根瘤菌靶向芳香族化合物。模式1的优越性能归因于曼氏硝化杆菌首先降解煤中的富氢结构，将大分子解聚成较小的芳香族化合物，并为慢生根瘤菌创造更多可利用的底物，从而改善了整体生物降解过程。相反，在模式2中，慢生根瘤菌在第一阶段主要降解富焦油煤中的多环芳烃，导致第二阶段可降解中间体减少，降低了降解过程的整体效率。这种顺序降解策略对其他类型的煤或有机污染物的适用性也值得探索，这可能会扩大其工业和环境相关性。
