Department of Civil and Environmental Engineering, Sung Kyun Kwan University, Jangan-Gu, Suwon, Gyeonggi-Do 440-746, Republic of Korea.
Mar Pollut Bull. 2011 Feb;62(2):388-94. doi: 10.1016/j.marpolbul.2010.09.020. Epub 2010 Oct 12.
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants in the environment and are derived from both man-made and natural resources. The present study is focused on the degradation of PAHs by a halotolerant bacterial strain under saline conditions. The bacterial strain VA1 was isolated from a PAH-degrading consortium that was enriched from marine water samples that were collected from different sites at Chennai, India. In the present study, a clearing zone formed on PAH-amended mineral salt agar media confirmed the utilization of PAH by the bacterial strain VA1. The results show that the strain VA1 was able to degrade anthracene (88%), phenanthrene (98%), naphthalene (90%), fluorene (97%), pyrene (84%), benzo(k)fluoranthene (57%) and benzo(e)pyrene (50%) at a 30 g/L NaCl concentration. The present study reveals that the VA1 strain was able to degrade PAHs in petroleum wastewater under saline conditions. The promising PAH-degrading halotolerant bacterial strain, VA1, was identified as Ochrobactrum sp. using biochemical and molecular techniques.
多环芳烃（PAHs）是环境中普遍存在的污染物，来源于人为和自然资源。本研究专注于在盐度条件下，耐盐细菌菌株对 PAHs 的降解。VA1 细菌菌株是从一个从印度钦奈不同地点采集的海洋水样中富集的 PAH 降解生物群落中分离出来的。在本研究中，在添加 PAH 的无机盐琼脂培养基上形成的透明圈证实了细菌菌株 VA1 对 PAH 的利用。结果表明，在 30 g/L NaCl 浓度下，菌株 VA1 能够降解蒽（88%）、菲（98%）、萘（90%）、芴（97%）、芘（84%）、苯并（k）荧蒽（57%）和苯并（e）芘（50%）。本研究表明，VA1 菌株能够在盐度条件下降解石油废水中的 PAHs。耐盐性好且具有 PAH 降解能力的细菌菌株 VA1 经生化和分子技术鉴定为食烷菌属（Ochrobactrum sp.）。
