Saito Rei, Katta Shiori, Takizawa Takeshi, Sugiyama Junichi, Kakizawa Yasushi, Morikawa Shigeru
Advanced Analytical Science Research Laboratories, Lion Corporation, 7-2-1 Hirai, Edogawa-ku, Tokyo, 132-0035, Japan.
Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, Imabari City, Ehime, Japan.
Sci Rep. 2025 Jul 1;15(1):21365. doi: 10.1038/s41598-025-05311-7.
Several studies on viral inactivation agents frequently overlook the influence of virus suspension conditions, which often leads to inconsistent conclusions. We investigated the impact of cell culture-derived media and environmental contaminants on the efficacy of viral inactivation agents against feline calicivirus (FCV). The FCV in Eagle's minimum essential medium (EMEM) with cell-derived metabolites, was replaced with distilled water (DW) with using a column-based simple dispersant replacement method. Sodium dodecyl sulfate (SDS) or sodium hypochlorite (NaClO) were less effective against FCV in EMEM than DW, while didecyl dimethylammonium chloride (DDAC) was more effective. The inactivation effect of 50% v/v ethanol was stronger against FCV in EMEM than DW, whereas that of 70% v/v ethanol was more effective in DW. Analysis of the effect of EMEM components revealed that inorganic salts and basic amino acids (BAA) reduced SDS inactivation efficacy but enhanced DDAC's. Similarly, inorganic salts reduced the effect of 70% v/v ethanol, while amino acids, especially BAA reduced that of NaClO's. Since each inactivating agent affects FCV differently, the influence of each EMEM component is assumed to differ. Moreover, environmental contaminants, such as bovine serum and saliva, reduced the overall effectiveness of the agents owing to their inorganic substances and proteins.
多项关于病毒灭活剂的研究常常忽视病毒悬液条件的影响，这往往导致结论不一致。我们研究了细胞培养衍生培养基和环境污染物对病毒灭活剂抗猫杯状病毒（FCV）效果的影响。采用基于柱的简单分散剂置换法，将含有细胞衍生代谢物的伊格尔氏最低必需培养基（EMEM）中的FCV用蒸馏水（DW）替代。十二烷基硫酸钠（SDS）或次氯酸钠（NaClO）在EMEM中对FCV的灭活效果不如在DW中，而双癸基二甲基氯化铵（DDAC）则更有效。50%（v/v）乙醇对EMEM中FCV的灭活作用比对DW中的更强，而70%（v/v）乙醇在DW中更有效。对EMEM成分作用的分析表明，无机盐和碱性氨基酸（BAA）降低了SDS的灭活效果，但增强了DDAC的效果。同样，无机盐降低了70%（v/v）乙醇的作用，而氨基酸，尤其是BAA降低了NaClO的作用。由于每种灭活剂对FCV的影响不同，推测EMEM各成分的影响也不同。此外，牛血清和唾液等环境污染物因其所含的无机物和蛋白质而降低了这些试剂的整体效果。
