Department of Food and Nutrition, Chung-Ang University, Anseong, Gyeonggi-Do 17546, Republic of Korea.
Department of Food and Nutrition, Chung-Ang University, Anseong, Gyeonggi-Do 17546, Republic of Korea.
Int J Food Microbiol. 2022 Feb 16;363:109506. doi: 10.1016/j.ijfoodmicro.2021.109506. Epub 2021 Dec 23.
A carrier (stainless steel disc as a default carrier) testing method is very needed for use in the actual food-processing fields by following the standard guideline. Here, we aimed to compare the virucidal efficacy of four commercial liquid disinfectants, including sodium hypochlorite (NaOCl), chlorine dioxide (ClO), and peracetic acid (PAA) against hepatitis A virus (HAV) following the OECD guideline protocol based on the quantitative carrier testing method and compared carrier testing results with the suspension testing results. The OECD method specifies a test for establishing whether a chemical disinfectant or a microbicide has a virucidal activity on hard non-porous surfaces. The antiviral efficacy was evaluated by plaque assays, and disinfectants were considered effective if the virus reduction was greater than or equal to 3 log (99.9% decrease) for carrier or 4 log (99.99% decrease) for suspension tests. Results indicated that ClO above 500 ppm and 50% ethanol were effective in the carrier test method. In contrast, more than 200 ppm NaOCl and 50 ppm ClO for all exposure times and 70% ethanol with contact for more than 5 min were effective in suspension tests. Treatment with PAA (80-2500 ppm) were not effective in carrier or suspension tests. Therefore, we recommend the use of more than 500 ppm ClO or 50% ethanol with exposure for 10 min to disinfect surfaces that may be contaminated with HAV. Thus, these results could be effective in establishing official antiviral efficacy testing methods and basic data.
需要遵循标准指南，为实际食品加工领域开发一种载体（默认载体为不锈钢盘）测试方法。在这里，我们旨在根据定量载体测试方法和悬浮测试结果比较四种商业液体消毒剂（次氯酸钠（NaOCl）、二氧化氯（ClO）和过氧乙酸（PAA））对甲型肝炎病毒（HAV）的杀病毒效果，比较载体测试结果和悬浮测试结果。OECD 方法规定了一种用于确定化学消毒剂或杀微生物剂对硬非多孔表面是否具有杀病毒活性的测试。通过噬菌斑测定评估抗病毒功效，如果载体测试中病毒减少大于或等于 3 对数（99.9%减少）或悬浮测试中病毒减少大于或等于 4 对数（99.99%减少），则认为消毒剂有效。结果表明，载体测试中 500ppm 以上的 ClO 和 50%乙醇有效。相比之下，在悬浮测试中，超过 200ppm 的 NaOCl 和 50ppm 的 ClO 持续暴露 10 分钟，以及 70%乙醇持续接触超过 5 分钟均有效。载体或悬浮测试中 PAA（80-2500ppm）处理无效。因此，我们建议使用 500ppm 以上的 ClO 或 50%乙醇，暴露 10 分钟，以消毒可能被 HAV 污染的表面。因此，这些结果可能有助于建立官方的抗病毒功效测试方法和基础数据。
