Jiangsu Provincial Center for Disease Prevention and Control, 172 Jiangsu Road, Nanjing, 210009, Jiangsu, China.
Key Laboratory of Enteric Pathogenic Microbiology, Ministry Health, Institute of Pathogenic Microbiology, 172 Jiangsu Road, Nanjing, 210009, China.
Sci Rep. 2021 Jan 28;11(1):2418. doi: 10.1038/s41598-021-82148-w.
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection is currently a global pandemic, and there are limited laboratory studies targeting pathogen resistance. This study aimed to investigate the effect of selected disinfection products and methods on the inactivation of SARS-CoV-2 in the laboratory. We used quantitative suspension testing to evaluate the effectiveness of the disinfectant/method. Available chlorine of 250 mg/L, 500 mg/L, and 1000 mg/L required 20 min, 5 min, and 0.5 min to inactivate SARS-CoV-2, respectively. A 600-fold dilution of 17% concentration of di-N-decyl dimethyl ammonium bromide (283 mg/L) and the same concentration of di-N-decyl dimethyl ammonium chloride required only 0.5 min to inactivate the virus efficiently. At 30% concentration for 1 min and 40% and above for 0.5 min, ethanol could efficiently inactivate SARS-CoV-2. Heat takes approximately 30 min at 56 °C, 10 min above 70 °C, or 5 min above 90 °C to inactivate the virus. The chlorinated disinfectants, Di-N-decyl dimethyl ammonium bromide/chloride, ethanol, and heat could effectively inactivate SARS-CoV-2 in the laboratory test. The response of SARS-CoV-2 to disinfectants is very similar to that of SARS-CoV.
严重急性呼吸系统综合症冠状病毒 2 型（SARS-CoV-2）感染目前是一种全球性的大流行，针对病原体耐药性的实验室研究有限。本研究旨在调查选定的消毒产品和方法对实验室中 SARS-CoV-2 灭活的效果。我们使用定量悬浮试验来评估消毒剂/方法的有效性。有效氯为 250mg/L、500mg/L 和 1000mg/L 时，分别需要 20 分钟、5 分钟和 0.5 分钟才能使 SARS-CoV-2 失活。17%浓度的双十二烷基二甲基溴化铵（283mg/L）稀释 600 倍和相同浓度的双十二烷基二甲基氯化铵仅需 0.5 分钟即可有效地使病毒失活。30%浓度作用 1 分钟、40%及以上浓度作用 0.5 分钟，乙醇即可有效灭活 SARS-CoV-2。在 56°C 下大约需要 30 分钟，在 70°C 以上需要 10 分钟，在 90°C 以上需要 5 分钟才能使病毒失活。氯化消毒剂、双十二烷基二甲基溴化铵/氯化物、乙醇和热在实验室测试中均可有效灭活 SARS-CoV-2。SARS-CoV-2 对消毒剂的反应与 SARS-CoV 非常相似。
