Han Li, Zhang Xinlong, Xu Hang, Xi Gaolei, Wang Ke, Chen Haoyang, Qi Wenyuan, Zhang Liuke, Huang Shen
Zhengzhou University of Light Industry, 136 Science Avenue, Zhengzhou High tech Zone, Zhengzhou, 450002, China.
Technology Center for China Tobacco Henan Industrial Limited Company, Zhengzhou, 450000, Henan, People's Republic of China.
Sci Rep. 2025 Jul 1;15(1):21824. doi: 10.1038/s41598-025-07179-z.
Cis-abienol, a naturally occurring labdanum diterpenoid, is believed to be an essential precursor of ambergris substances and is found on the surface of many plants. In this study, the strain Klebsiella oxytoca T2L was isolated from the soil of oriental tobacco and demonstrated effective degradation of cis-abienol. The strain was identified through morphological characteristics and 16 S rDNA sequence analysis. Under culture conditions of 30 °C, pH 7.0, 0.5 mg/mL ammonium sulfate, and 1 mg/mL cis-abienol-the degradation rate of cis-abienol by strain T2L reached 68.2% after 96 h. The influence of various carbon and nitrogen sources on the degradation effect of cis-abienol by T2L was examined. Additionally, the impact of pH and temperature on cell growth and cis-abienol degradation was also analyzed. GC-MS analysis indicated that the degradation of cis-abienol by strain T2L generated several potential aroma chemicals, including (+/-)-ambreinolide, sclareol, an analog of amberonne (isomer 3), and an analog of sclaral (sclareolide lactol), which resembled the composition of natural ambergris. This study provides a theoretical foundation for further research on the biodegradation of cis-abienol to high-value aroma compounds.
顺式秘鲁醇是一种天然存在的半日花烷二萜类化合物，被认为是龙涎香物质的重要前体，存在于许多植物的表面。在本研究中，从香料烟土壤中分离出一株产酸克雷伯菌T2L，并证明其对顺式秘鲁醇具有有效的降解作用。通过形态特征和16S rDNA序列分析对该菌株进行了鉴定。在30℃、pH 7.0、0.5mg/mL硫酸铵和1mg/mL顺式秘鲁醇的培养条件下，菌株T2L对顺式秘鲁醇的降解率在96小时后达到68.2%。考察了各种碳源和氮源对T2L降解顺式秘鲁醇效果的影响。此外，还分析了pH值和温度对细胞生长及顺式秘鲁醇降解的影响。气相色谱-质谱联用分析表明，菌株T2L对顺式秘鲁醇的降解产生了几种潜在的香气化学物质，包括（±）-龙涎香醇、硬尾醇、琥珀酮类似物（异构体3）和硬尾内酯类似物（硬尾醇内酯），这些物质与天然龙涎香的成分相似。本研究为进一步研究顺式秘鲁醇生物降解为高价值香气化合物提供了理论基础。
