The Experimental Forest, National Taiwan University, Nan-Tou 55750, Taiwan.
J Agric Food Chem. 2012 Jan 11;60(1):124-8. doi: 10.1021/jf2042196. Epub 2011 Dec 14.
The aims of the present study were to isolate and identify the antifungal compounds from the ethanolic extract of Cunninghamia konishii wood and to evaluate their antifungal activities against wood decay fungi. The results showed that the n-Hex soluble fraction of the ethanolic extract from C. konishii wood had an excellent inhibitory effect against Lenzites betulina, Trametes versicolor, Laetiporus sulphureus, and Gloeophyllum trabeum, with IC(50) values of 33, 46, 62, and 49 μg/mL, respectively. By following the bioactivity-guided fractionation procedure, four sesquiterpenes, T-cadinol, cedrol, T-muurolol, and (-)-epi-cedrol, and three diterpenes, 13-epi-manool, cis-abienol, and isoabienol, were identified from the active subfractions. Among the main constituents of the ethanolic extract from C. konishii, T-cadinol, cedrol, and T-muurolol efficiently inhibited the growth of four wood-rot fungi at the concentration of 100 μg/mL, with antifungal indices of 51.4-100.0%, 68.3-100.0%, and 39.5-100.0%, respectively. Results of this study show that the ethanolic extract of C. konishii wood may be considered as a potent source of T-cadinol, cedrol, and T-muurolol as new natural antifungal agents.
本研究的目的是从杉木木材的乙醇提取物中分离和鉴定抗真菌化合物，并评价其对木材腐朽真菌的抗真菌活性。结果表明，杉木乙醇提取物的正己烷可溶部分对栓孔菌、变色栓菌、硫黄菌和粗毛栓菌具有极好的抑制作用，IC50 值分别为 33、46、62 和 49μg/mL。通过生物活性导向的分段程序，从活性亚馏分中鉴定出了四种倍半萜类化合物，即 T-卡多醇、雪松醇、T-莫罗醇和（-）-表-雪松醇，以及三种二萜类化合物，13-表-曼诺醇、顺式-贝壳杉烯醇和异贝壳杉烯醇。在杉木乙醇提取物的主要成分中，T-卡多醇、雪松醇和 T-莫罗醇在 100μg/mL 浓度下有效地抑制了四种木材腐朽真菌的生长，其抑菌指数分别为 51.4-100.0%、68.3-100.0%和 39.5-100.0%。本研究结果表明，杉木木材的乙醇提取物可能被认为是 T-卡多醇、雪松醇和 T-莫罗醇作为新型天然抗真菌剂的潜在来源。
