Abbigeri Megha B, Khan Muskan, Thokchom Bothe, Singh Sapam Riches, Bhavi Santosh Mallikarjun, Kulkarni Sudheendra Rao, Harini B P, Reddy G Vidya Sagar, Govindasamy Chandramohan, Almutairi Khalid M, Babu Yarajarla Ramesh
Drosophila and Nanoscience Research Laboratory, Department of Applied Genetics, Karnatak University, Dharwad, Karnataka, 580003, India.
Department of Zoology, Bangalore University, Bengaluru, Karnataka, India.
Sci Rep. 2025 Jul 1;15(1):21572. doi: 10.1038/s41598-025-07780-2.
This study investigates the synthesis and characterization of Carbon dots (MA-CDs) derived from the aqueous extract of Martynia annua and examining their potential effects in an epilepsy model Drosophila melanogaster. Phytochemical analysis confirmed the presence of saponin, terpeniods, and flavanoids in the leaf extract, which facilitated the green synthesis of MA-CDs. Physicochemical characterization revealed an absorbance peak at 326 nm, the mean size of the particle was 3.17 ± 0.16 nm, and moderate stability (-1.6 mV). To assess the therapeutic potential of MA-CDs alongside the antiepileptic drug Carbamazepine (CBZ), we conducted behavioral and cognitive assays in para bang senseless (para) mutants of Drosophila, a model organism for epilepsy. Seizures induced by vortex and heat shock were significantly mitigated in a dose-dependent manner in flies treated with both MA-CDs and CBZ. However, higher doses of CBZ and MA-CDs increased the climbing ability of the flies. In cognitive assays, CBZ at higher doses improved memory and learning in mutant flies, while MA-CDs also showed significant impact. MA-CDs were consumed at a higher rate than CBZ when incorporated into food. The green synthesized MA-CDs at its higher concentration has garnered its positive effect on the mutants along with the CBZ antiepileptic drug which also has shown its positive effects when different concentration of them were treated to the mutants.
本研究调查了从一年生角胡麻的水提取物中合成并表征的碳点（MA-CDs），并研究了它们在癫痫模型果蝇中的潜在作用。植物化学分析证实叶提取物中存在皂苷、萜类化合物和黄酮类化合物，这促进了MA-CDs的绿色合成。物理化学表征显示在326nm处有一个吸收峰，颗粒平均尺寸为3.17±0.16nm，稳定性适中（-1.6mV）。为了评估MA-CDs与抗癫痫药物卡马西平（CBZ）的治疗潜力，我们在癫痫模型生物果蝇的无义突变体（para）中进行了行为和认知测定。在用MA-CDs和CBZ处理的果蝇中，由涡旋和热休克诱导的癫痫发作以剂量依赖性方式显著减轻。然而，较高剂量的CBZ和MA-CDs提高了果蝇的攀爬能力。在认知测定中，较高剂量的CBZ改善了突变果蝇的记忆和学习能力，而MA-CDs也显示出显著影响。当掺入食物中时，MA-CDs的消耗速度高于CBZ。绿色合成的高浓度MA-CDs与CBZ抗癫痫药物一起对突变体产生了积极影响，当将不同浓度的它们处理给突变体时，CBZ也显示出了积极作用。
