干旱和低温胁迫下蟋蟀草TCP基因家族的全基因组鉴定及胁迫响应表达谱分析

Genome-wide identification and stress-responsive expression profiling of the TCP gene family in Cenchrus fungigraminus under drought and cold stress.

作者信息

Ke Shijie, He Xin, Zhang Mengmeng, Luo Lin, Chen Yuxin, Cui Yixue, Huang Yelin

机构信息

State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Stress Biology, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.

College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

出版信息

BMC Genomics. 2025 Jul 1;26(1):626. doi: 10.1186/s12864-025-11798-1.

DOI:10.1186/s12864-025-11798-1

PMID:40596840

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12211166/

Abstract

BACKGROUND

TCP transcription factors play a crucial role in various biological processes, including plant growth, development, and response to abiotic stress. However, few related studies investigated the characteristics of TCP genes of and how it plays a role in abiotic stress responses.

RESULTS

Here, we identified 40 unevenly distributed across 13 chromosomes of and classified them into three subfamilies based on the conserved domain and phylogenetic analysis. Sequence analysis revealed that all contain basic Helix-Loop-Helix conserved regions, and the majority of the genes were intronless. Twelve genes in the CIN subclade had potential miR319 target sites. Cis-acting element analysis showed that most genes contained many light-, phytohormone-, and developmental stress-responsive elements in their promoter regions. Furthermore, play an important role in biological activities such as transcription regulation, regulation of biosynthetic processes, and metabolic processes, and were involved in plant circadian rhythms and environmental adaptation pathways. Notably, RNA-seq data showed that most were involved in stress response under drought and cold treatments. Through RNA-seq analysis and qPCR validation, were found to play a positive regulatory role in drought stress, while plays a negative potentially.

CONCLUSIONS

In total, 40 genes were identified in . Functional predictions suggested the roles of in modulating multiple physiological processes and metabolic pathways. The induced expression of under drought and cold stress indicated their involvement in abiotic stress response in . These findings lay the groundwork for further research on TCP genes in .

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1186/s12864-025-11798-1.

摘要

背景

TCP转录因子在包括植物生长、发育以及对非生物胁迫的响应等多种生物学过程中发挥着关键作用。然而，很少有相关研究探究[具体植物名称]的TCP基因特征以及它在非生物胁迫响应中如何发挥作用。

结果

在此，我们在[具体植物名称]的13条染色体上鉴定出40个[具体基因名称]，它们分布不均，并基于保守结构域和系统发育分析将其分为三个亚家族。序列分析表明，所有[具体基因名称]都包含基本的螺旋-环-螺旋保守区域，并且大多数[具体基因名称]基因无内含子。CIN亚分支中的12个基因具有潜在的miR319靶位点。顺式作用元件分析表明，大多数[具体基因名称]基因在其启动子区域含有许多光、植物激素和发育胁迫响应元件。此外，[具体基因名称]在转录调控、生物合成过程调控和代谢过程等生物学活动中发挥重要作用，并参与植物昼夜节律和环境适应途径。值得注意的是，RNA测序数据表明，大多数[具体基因名称]在干旱和寒冷处理下参与胁迫响应。通过RNA测序分析和qPCR验证，发现[具体基因名称]在干旱胁迫中起正调控作用，而[具体基因名称]可能起负调控作用。