Hoveizi Elham, Naddaf Hadi, Ahmadianfar Sina, Gutmann James Leo
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Clinical Sciences, Faculty of Veterinary, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
J Biosci Bioeng. 2023 Apr;135(4):331-340. doi: 10.1016/j.jbiosc.2022.12.009. Epub 2023 Jan 26.
This study aimed to determine the impact of human endometrial stem cells (EnSCs) and titanium oxide nanoparticles (TiO NPs) on dental pulp repair and regeneration in an animal model through dentine development and tissue regeneration. The EnSCs were put on a three-dimensional (3D) chitosan scaffold containing TiO NPs after obtaining and purifying the collagenase enzyme. Pulps were exposed on the maxillary left first molar of all rats followed by direct pulp capping with the experimental scaffolds, as follows. Groups were: 1, control group without any treatment; 2, chitosan group (CS); 3, chitosan group with stem cells (CS/SCs); 4, chitosan group with stem cells and TiO NPs (CS/EnSCs/TiO). Glass ionomer was used as a sealant in all groups. The teeth were extracted and histologically evaluated after 8 weeks. The quality and amount of dentine in the CS/EnSCs/TiO group were higher than in the other groups. The combination of EnSCs with TiO NPs and 3D chitosan scaffolds had a synergistic effect on each other, evidencing increased speed and quality of dentine formation. Using EnSCs with TiO NPs on a 3D chitosan scaffold can be a suitable combination for direct pulp capping and dentine regeneration in a rat molar tooth model.
本研究旨在通过牙本质发育和组织再生，在动物模型中确定人子宫内膜干细胞（EnSCs）和二氧化钛纳米颗粒（TiO NPs）对牙髓修复和再生的影响。在获得并纯化胶原酶后，将EnSCs置于含有TiO NPs的三维（3D）壳聚糖支架上。对所有大鼠的上颌左侧第一磨牙进行牙髓暴露，然后用实验性支架进行直接盖髓，具体分组如下：1组为未进行任何处理的对照组；2组为壳聚糖组（CS）；3组为含干细胞的壳聚糖组（CS/SCs）；4组为含干细胞和TiO NPs的壳聚糖组（CS/EnSCs/TiO）。所有组均使用玻璃离子水门汀作为封闭剂。8周后拔牙并进行组织学评估。CS/EnSCs/TiO组的牙本质质量和数量高于其他组。EnSCs与TiO NPs和3D壳聚糖支架之间存在协同作用，证明牙本质形成的速度和质量均有所提高。在大鼠磨牙模型中，将EnSCs与TiO NPs用于3D壳聚糖支架可作为直接盖髓和牙本质再生的合适组合。
