Taghavinia Fatemeh, Taghehchian Negin, Salahshourifar Iman, Abbaszadegan Mohammad Reza
Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
BMC Med Genomics. 2025 Jul 1;18(1):109. doi: 10.1186/s12920-025-02156-x.
Long-chain non-coding RNAs (lncRNAs) play various roles in the regulation of gene expression at the levels of transcription and translation, and epigenetic modification. Dysregulation of lncRNAs is associated with various malignancies, including cancer. lncRNAs have been demonstrated to regulate critical biological processes in cancer cells, such as apoptosis, proliferation, migration, and invasion. They also play essential roles in the development of gastric cancer (GC). However, the clinical significance and biological function of many lncRNAs remain unexplored in GC progression. This study aimed to evaluate the expression profiles of LINC00671 and LINC01913 in GC patients and investigate their correlation with epithelial-to-mesenchymal transition (EMT) markers.
The real-time PCR technique was applied to measure the expression levels of the selected lncRNAs (LINC01913 and LINC00671) and EMT-related mRNAs (MAMLs and MMP-13) in 83 tumor and adjacent normal tissues obtained from GC patients.
A significant reduction in LINC00671 expression was observed in 55.4% of tumor tissues, while elevated expression of LINC01913 (41%), MMP13 (56.6%), and MAML1 (44.6%) was detected, representing the proportion of samples with dysregulated expression relative to matched normal tissues. Dysregulation of these genes was significantly associated with various clinicopathological features (P < 0.05), supporting a potential link between these lncRNAs and EMT processes in GC.
The observed associations between LINC00671, LINC01913, and EMT-related genes suggest their potential as prognostic biomarkers for treatment response in GC patients.
长链非编码RNA（lncRNAs）在转录、翻译及表观遗传修饰水平的基因表达调控中发挥着多种作用。lncRNAs失调与包括癌症在内的多种恶性肿瘤相关。lncRNAs已被证明可调节癌细胞中的关键生物学过程，如凋亡、增殖、迁移和侵袭。它们在胃癌（GC）的发生发展中也起着重要作用。然而，许多lncRNAs在GC进展中的临床意义和生物学功能仍未被探索。本研究旨在评估LINC00671和LINC01913在GC患者中的表达谱，并研究它们与上皮-间质转化（EMT）标志物的相关性。
应用实时PCR技术检测从GC患者获取的83个肿瘤组织及相邻正常组织中所选lncRNAs（LINC01913和LINC00671）和EMT相关mRNA（MAMLs和MMP-13）的表达水平。
在55.4%的肿瘤组织中观察到LINC00671表达显著降低。同时检测到LINC01913（41%）、MMP13（56.6%）和MAML1（44.6%）表达升高，这些比例代表了相对于匹配正常组织而言表达失调的样本比例。这些基因的失调与多种临床病理特征显著相关（P < 0.05），支持了这些lncRNAs与GC中EMT过程之间的潜在联系。
观察到的LINC00671、LINC01913与EMT相关基因之间的关联表明，它们有可能作为GC患者治疗反应的预后生物标志物。
