Jin Wen, Jia Jianchao, Si Yangming, Liu Jianli, Li Hanshuang, Zhu Hao, Wu Zhouying, Zuo Yongchun, Yu Lan
Clinical Medical Research Center, Inner Mongolia Key Laboratory of Gene Regulation of the Metabolic Disease, Inner Mongolia People's Hospital, Hohhot, 010010, China.
Laboratory of Theoretical Biophysics, School of Physical Science and Technology, Inner Mongolia University, Hohhot, 010021, China.
Biochem Genet. 2024 Apr 24. doi: 10.1007/s10528-024-10801-w.
Long non-coding RNAs (lncRNAs), as promising novel biomarkers for cancer treatment and prognosis, can function as tumor suppressors and oncogenes in the occurrence and development of many types of cancer, including gastric cancer (GC). However, little is known about the complex regulatory system of lncRNAs in GC. In this study, we systematically analyzed lncRNA and miRNA transcriptomic profiles of GC based on bioinformatics methods and experimental validation. An lncRNA-miRNA interaction network related to GC was constructed, and the nine crucial lncRNAs were identified. These 9 lncRNAs were found to be associated with the prognosis of GC patients by Cox proportional hazards regression analysis. Among them, the expression of lncRNA SNHG14 can affect the survival of GC patients as a potential prognostic marker. Moreover, it was shown that SNHG14 was involved in immune-related pathways and significantly correlated with immune cell infiltration in GC. Meanwhile, we found that SNHG14 affected immune function in many cancers, such as breast cancer and esophageal carcinoma. Such information revealed that SNHG14 may serve as a potential target for cancer immunotherapy. As well, our study could provide practical and theoretical guiding significance for clinical application of non-coding RNAs.
长链非编码RNA（lncRNAs）作为癌症治疗和预后颇具前景的新型生物标志物，在包括胃癌（GC）在内的多种癌症的发生和发展过程中可发挥肿瘤抑制因子和癌基因的作用。然而，关于lncRNAs在GC中的复杂调控系统，我们知之甚少。在本研究中，我们基于生物信息学方法和实验验证，系统地分析了GC的lncRNA和miRNA转录组图谱。构建了与GC相关的lncRNA-miRNA相互作用网络，并鉴定出9个关键lncRNAs。通过Cox比例风险回归分析发现，这9个lncRNAs与GC患者的预后相关。其中，lncRNA SNHG14的表达作为一种潜在的预后标志物，可影响GC患者的生存。此外，研究表明SNHG14参与免疫相关途径，且与GC中的免疫细胞浸润显著相关。同时，我们发现SNHG14在许多癌症中影响免疫功能，如乳腺癌和食管癌。这些信息表明，SNHG14可能作为癌症免疫治疗的潜在靶点。同样，我们的研究可为非编码RNA的临床应用提供实践和理论指导意义。
