Soltani Borchaloee A, Moosakazemi Mohammadi L S, Khosh Ravesh R, Allameh S F, Tabatabaie Poya F S, Fatehi Marj A
Saba Biomedicals Science-Based Company, Tehran, Iran.
Department of Biology, Faculty of Basic Science, Semnan University, Semnan, Iran.
Arch Razi Inst. 2024 Dec 31;79(6):1281-1286. doi: 10.32592/ARI.2024.79.6.1281. eCollection 2024 Dec.
Pseudomonas aeruginosa is a significant pathogen responsible for nosocomial infections. P. aeruginosa is a multidrug-resistant (MDR) bacterium that is postulated to be the result of its plasmid-borne and intrinsic resistance to a number of pharmaceutical agents. This study examined the potential for biofilm formation, the distribution of the pslD, pelF, and algD genes, and the expression of the MexAB-OprM efflux pump genes. Furthermore, the study examined the pattern of antibiotic resistance in multi-drug resistant P. aeruginosa isolates obtained from a range of clinical samples. A total of 76 strains of P. aeruginosa were obtained for this investigation from a range of clinical specimens. The susceptibility of the isolates to antibiotics was evaluated using the disk agar diffusion method. In conclusion, the term "multi-drug resistance" (MDR) is used to describe a specific pattern of resistance. The isolates were evaluated for the presence of three pivotal biofilm genes and their antimicrobial resistance patterns against ten standard antibiotic disks. The data were analyzed using version 25 of the SPSS statistical software. The examination of the isolates revealed that the most antibiotic sensitivity was associated with polymyxin, piperacillin, and ciprofloxacin. Additionally, the prevalence of biofilm-producing genes, specifically pslD, pelF, and algD, was determined to be 68.4%, 80.3%, and 69.7%, respectively. The prevalence of MexAB-OprM efflux genes in the examined isolates was 89.5% for the mexA gene, 90.8% for the mexB gene, and 90.8% for the oprM gene. The majority of the isolates in this investigation exhibited the presence of efflux pump genes, as evidenced by the findings. Furthermore, a robust correlation was identified between a select number of efflux genes and biofilm formation or the antibiotics tetracycline, meropenem, amikacin, and polymyxin B.
铜绿假单胞菌是引起医院感染的重要病原体。铜绿假单胞菌是一种多重耐药（MDR）细菌，据推测这是其质粒介导的以及对多种药剂的固有耐药性导致的结果。本研究检测了生物膜形成的可能性、pslD、pelF和algD基因的分布以及MexAB - OprM外排泵基因的表达。此外，该研究还检测了从一系列临床样本中分离出的多重耐药铜绿假单胞菌菌株的抗生素耐药模式。本研究共从一系列临床标本中获取了76株铜绿假单胞菌菌株。采用纸片琼脂扩散法评估分离菌株对抗生素的敏感性。总之，“多重耐药”（MDR）一词用于描述一种特定的耐药模式。对分离菌株检测了三个关键生物膜基因的存在情况以及它们对十种标准抗生素纸片的抗菌耐药模式。使用SPSS统计软件25版对数据进行分析。对分离菌株的检测表明，最敏感的抗生素是多粘菌素、哌拉西林和环丙沙星。此外，生物膜产生基因，特别是pslD、pelF和algD的流行率分别确定为68.4%、80.3%和69.7%。在所检测的分离菌株中，MexAB - OprM外排基因的流行率为：mexA基因89.5%，mexB基因90.8%，oprM基因90.8%。本研究中的大多数分离菌株表现出存在外排泵基因，研究结果证明了这一点。此外，还发现了一些外排基因与生物膜形成或抗生素四环素、美罗培南、阿米卡星和多粘菌素B之间存在强相关性。
