Bahador Nima, Shoja Saeed, Faridi Foroogh, Dozandeh-Mobarrez Banafsheh, Qeshmi Fatemeh Izadpanah, Javadpour Sedigheh, Mokhtary Sedigheh
Department of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Iran J Microbiol. 2019 Feb;11(1):25-30.
is a ubiquitous opportunistic pathogen. The presence of several virulence factors such as exotoxin and exoenzyme genes and biofilm may contribute to its pathogenicity. The purpose of this study was to investigate the presence of and , the determination of biofilm production and antimicrobial susceptibility patterns among clinical isolates of .
In this study, 75 isolates of were recovered from various clinical specimens. Antimicrobial susceptibility pattern of isolates were identified. Virulence genes and were determined using PCR. The ability of biofilm production was assessed.
Antimicrobial susceptibility test showed that 12 strains were resistant to more than 8 antibiotics (17.14%). The most effective antibiotic was colistin as 98.6% of isolates were sensitive. The frequencies of and genes were detected as 36.6% and 55.7%, respectively. In addition, 98.6% of the isolates were biofilm producers. Exotoxin A was detected in sixty-eight isolates (95.7%).
The findings of this study showed that, the presence of exotoxin and exoenzyme genes, particularly, the gene is the most common virulence factors in the bacterial isolates from urine samples. Biofilm is a serious challenge in the treatment of infection.
[病原体名称]是一种普遍存在的机会致病菌。外毒素、外酶基因和生物膜等多种毒力因子的存在可能导致其致病性。本研究旨在调查[病原体名称]临床分离株中[毒力因子名称1]和[毒力因子名称2]的存在情况，确定生物膜的产生情况以及抗菌药物敏感性模式。
在本研究中，从各种临床标本中分离出75株[病原体名称]。鉴定分离株的抗菌药物敏感性模式。使用聚合酶链反应（PCR）测定毒力基因[毒力因子名称1]和[毒力因子名称2]。评估生物膜产生能力。
抗菌药物敏感性试验表明，12株菌株对8种以上抗生素耐药（17.14%）。最有效的抗生素是黏菌素，98.6%的分离株对其敏感。[毒力因子名称1]和[毒力因子名称]基因的检出频率分别为36.6%和55.7%。此外，98.6%的分离株是生物膜产生菌。68株（95.7%）分离株检测到外毒素A。
本研究结果表明，外毒素和外酶基因的存在，特别是[毒力因子名称2]基因是尿液样本细菌分离株中最常见的毒力因子。生物膜是[病原体名称]感染治疗中的一个严峻挑战。
