Garcia Joseph A, Vergara Mendez Ashley, Sletten Ellen M
Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.
Macromolecules. 2024 Jul 9;57(13):6354-6361. doi: 10.1021/acs.macromol.4c00324. Epub 2024 Jun 24.
Biotinylated hydrophilic polymers are used in a plethora of chemical and biochemical assays due to the exceptional affinity of biotin for (strept)avidin. Here, we report a facile method for the installation of biotin at the end of poly(oxazoline)s (P(Ox)) using an electrophilic biotin initiator. While this method is applicable to many different oxazoline monomers, we focus on biotin-poly(2-methyl-2-oxazoline) as it is a desirable alternative to poly(ethylene glycol) (PEG), which has rising immunogenicity concerns. A further advantage of P(Ox) over PEG is the high degree of chemical flexibility that can be imparted, which we showcase through the preparation of varied molecular weight, amphiphilic, and bifunctional P(Ox)s. Furthermore, we demonstrate the high efficacy of these biotin polymers through a series of common biochemical assays that frequently utilize the biotin-streptavidin pair.
由于生物素对（链）抗生物素蛋白具有特殊的亲和力，生物素化的亲水性聚合物被广泛应用于大量的化学和生化分析中。在此，我们报道了一种使用亲电生物素引发剂在聚恶唑啉（P(Ox)）末端安装生物素的简便方法。虽然这种方法适用于许多不同的恶唑啉单体，但我们专注于生物素化聚（2-甲基-2-恶唑啉），因为它是聚乙二醇（PEG）的理想替代品，而聚乙二醇的免疫原性问题日益受到关注。与聚乙二醇相比，聚恶唑啉的另一个优点是可以赋予高度的化学灵活性，我们通过制备不同分子量、两亲性和双功能的聚恶唑啉来展示这一点。此外，我们通过一系列经常使用生物素-链霉抗生物素蛋白对的常见生化分析，证明了这些生物素聚合物的高效性。
