Dehghanzad Reyhaneh, Rahbar Parvaneh Roghayeh, Keramatipour Mohammad, Kadkhoda Sepideh, Aghajanpour Mohsen, Taslimi Reza, Sahebi Leyla, Shakoori Farahani Abbas
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Cell J. 2024 Dec 1;26(12):700-710. doi: 10.22074/cellj.2025.2042114.1684.
Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancerrelated deaths worldwide, posing a significant public health challenge. Recent advances in molecular research and technologies have highlighted the potential of long non-coding RNAs (lncRNAs) as key players in CRC development and progression. High-throughput technologies, such as RNA sequencing (RNA-seq), enable comprehensive analysis of cancer omics data, providing valuable insights into CRC biology. This study aimed to identify and validate lncRNAs with potential roles in CRC pathogenesis.
In this experimental study, we conducted RNA-seq analysis using data from The Cancer Genome Atlas (TCGA) to identify differentially expressed lncRNAs between 481 CRC and 41 healthy control samples. Using the Boruta feature selection algorithm, we identified statistically significant lncRNAs. Based on a comprehensive literature review, four lncRNAs ( and ) were selected for experimental validation. Real-time reverse transcription polymerase chain reaction (RT-PCR) was performed on 10 primary CRC and 9 normal colorectal tissue samples to quantify the expression of these lncRNAs. Additionally, bioinformatic analyses were conducted to explore molecular pathways associated with these lncRNAs in CRC.
RNA-seq analysis identified 388 differentially expressed lncRNAs in CRC tissues compared to healthy controls. Among these, four lncRNAs ( and ) were validated through real-time RT-PCR, confirming their significant upregulation in CRC tissues. Bioinformatic analysis revealed their potential involvement in molecular pathways critical to tumor growth and metastasis.
This study identified and validated four significantly upregulated lncRNAs (, and ) in CRC tissues, providing evidence of their potential roles in CRC pathogenesis. These lncRNAs could serve as promising candidates for future biomarker development and therapeutic research in colorectal cancer.
结直肠癌（CRC）是全球第三大常见癌症，也是癌症相关死亡的第二大主要原因，对公共卫生构成重大挑战。分子研究和技术的最新进展凸显了长链非编码RNA（lncRNAs）在CRC发生发展中作为关键角色的潜力。高通量技术，如RNA测序（RNA-seq），能够对癌症组学数据进行全面分析，为CRC生物学提供有价值的见解。本研究旨在鉴定和验证在CRC发病机制中具有潜在作用的lncRNAs。
在本实验研究中，我们使用来自癌症基因组图谱（TCGA）的数据进行RNA-seq分析，以鉴定481例CRC样本和41例健康对照样本之间差异表达的lncRNAs。使用Boruta特征选择算法，我们确定了具有统计学意义的lncRNAs。基于全面的文献综述，选择了四个lncRNAs（和）进行实验验证。对10例原发性CRC组织样本和9例正常结直肠组织样本进行实时逆转录聚合酶链反应（RT-PCR），以定量这些lncRNAs的表达。此外，还进行了生物信息学分析，以探索这些lncRNAs在CRC中相关的分子途径。
与健康对照相比，RNA-seq分析在CRC组织中鉴定出388个差异表达的lncRNAs。其中，四个lncRNAs（和）通过实时RT-PCR得到验证，证实它们在CRC组织中显著上调。生物信息学分析揭示了它们可能参与对肿瘤生长和转移至关重要的分子途径。
本研究鉴定并验证了CRC组织中四个显著上调的lncRNAs（、和），证明了它们在CRC发病机制中的潜在作用。这些lncRNAs有望成为未来结直肠癌生物标志物开发和治疗研究的候选对象。
