Gonçalves Letícia Boslooper, Trevisan Andrea, Franco Eduardo L, Villa Luisa L, Trottier Helen, de Araujo-Souza Patrícia Savio
Post-Graduation Program in Genetics, Department of Genetics, Universidade Federal do Paraná (UFPR), Curitiba, Brazil.
Laboratory of Immunogenetics and Histocompatibility, Department of Genetics, Universidade Federal do Paraná (UFPR), Curitiba, Brazil.
J Med Virol. 2025 Jul;97(7):e70471. doi: 10.1002/jmv.70471.
Little is known about the factors associated with the natural humoral immune response against human papillomavirus (HPV) infection. The association between HLA-DRB1 and -DQB1 polymorphism and (1) HPV16 DNA detection, (2) naturally developed humoral immune response (IgG and IgM) against HPV16 L1 and L2 capsid proteins, and (3) type of HPV16 infection (transient or persistent) was investigated. Data from 943 women participating in the Ludwig-McGill cohort study was analyzed. HLA-DRB1 and HLA-DQB1 genotyping was done by PCR-based methods. HPV DNA was assessed every 4 months during the first year of follow-up, and HPV16 IgG and IgM antibody measurements were performed by ELISA in serum samples from the first and/or second visits. Associations were estimated by adjusted logistic regression models. HLA-DRB103:02 was associated with HPV16 DNA positivity (OR = 2.49, 95% CI: 1.11-5.60). HLA-DRB113:01 and HLA-DQB1*04:02 were associated with low levels of HPV16 IgG antibodies (OR = 0.44, 95% CI:0.23-0.87; OR = 0.10, 95% CI: 0.02-0.55, respectively). Neither IgM HPV16 antibodies nor the type of HPV16 infection (transient or persistent) were associated with HLA-DRB1 or -DQB1 variants. HLA-DRB1 and -DQB1 polymorphism might be associated with different levels of IgG against HPV16 infection, but IgM production and the type of infection seem to occur independently of these variants.
关于与针对人乳头瘤病毒（HPV）感染的天然体液免疫反应相关的因素，人们了解甚少。研究了HLA - DRB1和 - DQB1基因多态性与（1）HPV16 DNA检测、（2）针对HPV16 L1和L2衣壳蛋白的自然产生的体液免疫反应（IgG和IgM）以及（3）HPV16感染类型（短暂性或持续性）之间的关联。分析了参与路德维希 - 麦吉尔队列研究的943名女性的数据。通过基于PCR的方法进行HLA - DRB1和HLA - DQB1基因分型。在随访的第一年中，每4个月评估一次HPV DNA，并通过ELISA对首次和/或第二次就诊时采集的血清样本进行HPV16 IgG和IgM抗体检测。通过调整后的逻辑回归模型估计关联。HLA - DRB103:02与HPV16 DNA阳性相关（OR = 2.49，95% CI：1.11 - 5.60）。HLA - DRB113:01和HLA - DQB1*04:02与低水平的HPV16 IgG抗体相关（OR分别为0.44，95% CI：0.23 - 0.87；OR = 0.10，95% CI：0.02 - 0.55）。HPV16 IgM抗体和HPV16感染类型（短暂性或持续性）均与HLA - DRB1或 - DQB1变体无关。HLA - DRB1和 - DQB1基因多态性可能与针对HPV16感染的不同水平的IgG相关，但IgM的产生和感染类型似乎独立于这些变体发生。
