Nuño-Soriano Ana, Arias-Hidalgo Carlota, Juanes-Velasco Pablo, Gongora Rafael, Hernández Angela-Patricia, Fuentes Manuel
Department of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), IBSAL, Universidad de Salamanca-CSIC, Salamanca, Spain.
Proteomics Unit, Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain.
Methods Mol Biol. 2025;2929:215-226. doi: 10.1007/978-1-0716-4595-6_16.
Immunogenic Cell Death (ICD) is a type of cell death regulated by an activation of endoplasmic reticulum stress. This signal results in an activation of the adaptative immune response against the affected cell. This characteristic has been used for the design of novel oncological drugs to complement antitumor therapy with this immunogenic response. However, ICD is complex and still needs a deep and massive study to unravel the key to its mechanism. Protein microarrays are a useful tool for large-scale protein detection and analysis, and thus may be the suitable platform for this study. In this study, antibody arrays have been designed and constructed as a method to detect different proteins involved in programmed cell death pathways in order to evaluate differential intracellular signalling profiles and correlate them with ICD.
免疫原性细胞死亡（ICD）是一种由内质网应激激活所调控的细胞死亡类型。该信号会引发针对受影响细胞的适应性免疫反应激活。这一特性已被用于新型肿瘤药物的设计，以通过这种免疫原性反应来补充抗肿瘤治疗。然而，ICD较为复杂，仍需要深入且大量的研究来揭示其机制的关键所在。蛋白质微阵列是用于大规模蛋白质检测和分析的有用工具，因此可能是这项研究的合适平台。在本研究中，已设计并构建了抗体阵列，作为一种检测参与程序性细胞死亡途径的不同蛋白质的方法，以便评估细胞内信号传导差异图谱，并将它们与ICD相关联。
