Takahashi O, Sakamoto Y, Hiraga K
Toxicol Lett. 1985 Sep;27(1-3):15-25. doi: 10.1016/0378-4274(85)90115-8.
Male Sprague-Dawley rats were injected intraperitoneally (i.p.) with butylated hydroxyanisole (BHA) at doses of 0, 1, 4, 16, 64, 256, 384, 576, 864, 1296 and 1944 mg/kg/day for 7 days. Deaths occurred in a dose- and time-dependent manner when BHA was given in amounts greater than 576 mg/kg. The LD50 was 881 (484-1440) mg/kg. Intracranial hemorrhage was found in the dead rats, and lung hemorrhage was observed in the survivors given BHA at doses greater than 384 mg/kg/day. Histopathologically, intra-alveolar hemorrhages, thickening of alveoli and deposition of lipids in the lungs were observed. The prothrombin index was decreased only in rats given BHA at a level of 384 mg/kg/day. These observations suggest that BHA and/or its metabolite cause pulmonary hemorrhagic damage in rats. The mechanism of hemorrhage may be different from that of bleeding induced by butylated hydroxytoluene.
将雄性斯普拉格-道利大鼠腹腔注射丁基羟基茴香醚（BHA），剂量分别为0、1、4、16、64、256、384、576、864、1296和1944毫克/千克/天，持续7天。当BHA剂量大于576毫克/千克时，死亡呈剂量和时间依赖性。半数致死量为881（484 - 1440）毫克/千克。在死亡大鼠中发现颅内出血，在接受BHA剂量大于384毫克/千克/天的存活大鼠中观察到肺出血。组织病理学检查发现，肺内有肺泡内出血、肺泡增厚和脂质沉积。仅在接受384毫克/千克/天BHA剂量的大鼠中，凝血酶原指数降低。这些观察结果表明，BHA和/或其代谢产物会对大鼠造成肺出血性损伤。出血机制可能与丁基羟基甲苯诱导的出血机制不同。
