Sevindik Mustafa, Krupodorova Tetiana, Uysal İmran, Koçer Oguzhan, Sevindik Emre
Department of Biology, Faculty of Engineering and Natural Sciences, University of Osmaniye Korkut Ata, Osmaniye, 80000, Türkiye.
Department of Plant Food Products and Biofortification, Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, 04123, Ukraine.
Sci Rep. 2025 Jul 2;15(1):23431. doi: 10.1038/s41598-025-08936-w.
Visnaga daucoides (Desf.) Celak. (Apiaceae) is a plant known for its medicinal properties, but its phenolic content and biological activities remain underexplored, especially in relation to samples from different geographical regions. Exploring how environmental conditions may influence the plant's bioactive profile can provide valuable insights for its potential medicinal use. This study investigates the phenolic profile, antioxidant, anticholinesterase, and antiproliferative activities of V. daucoides collected from Iraq and Türkiye. In this study, the phenolic composition of Visnaga daucoides was determined by LC-MS/MS analysis. Antioxidant status was evaluated using TAS, TOS and OSI analyses, while antiproliferative activity was evaluated by MTT method on A549 lung cancer cells. Anticholinesterase activity was measured using Ellman method and antimicrobial activity was tested using agar dilution analysis. LC-MS/MS analysis revealed significant phenolic compounds, including acetohydroxamic acid, kaempferol, quercetin, gallic acid, and resveratrol, with geographical differences observed between the two regions. The plant exhibited potent antioxidant activity, as demonstrated by variations in total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI), which were determined using Rel Assay diagnostic kits. Furthermore, V. daucoides displayed notable antiproliferative effects, particularly against A549 lung cancer cells, and strong anticholinesterase activity, with inhibition of both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). These findings suggest that V. daucoides is a promising source of bioactive compounds with potential applications in cancer therapy, neuroprotection, and oxidative stress management. The study also emphasizes the influence of environmental factors on the chemical composition and biological activities of the plant, warranting further investigation into its pharmacological potential for therapeutic use.
紫花前胡（Visnaga daucoides (Desf.) Celak.，伞形科）是一种因其药用特性而闻名的植物，但其酚类成分和生物活性仍未得到充分研究，特别是与来自不同地理区域的样本相关的研究较少。探索环境条件如何影响该植物的生物活性特征可为其潜在药用价值提供有价值的见解。本研究调查了从伊拉克和土耳其采集的紫花前胡的酚类特征、抗氧化、抗胆碱酯酶和抗增殖活性。在本研究中，通过液相色谱-串联质谱（LC-MS/MS）分析确定了紫花前胡的酚类成分。使用总抗氧化能力（TAS）、总氧化能力（TOS）和氧化应激指数（OSI）分析评估抗氧化状态，同时通过MTT法对A549肺癌细胞评估抗增殖活性。使用Ellman法测量抗胆碱酯酶活性，并使用琼脂稀释分析法测试抗菌活性。LC-MS/MS分析揭示了包括乙酰氧肟酸、山奈酚、槲皮素、没食子酸和白藜芦醇在内的重要酚类化合物，且在两个地区观察到了地理差异。该植物表现出强大的抗氧化活性，通过使用Rel Assay诊断试剂盒测定的总抗氧化状态（TAS）、总氧化状态（TOS）和氧化应激指数（OSI）的变化得以证明。此外，紫花前胡显示出显著的抗增殖作用，特别是对A549肺癌细胞，以及强大的抗胆碱酯酶活性，对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）均有抑制作用。这些发现表明紫花前胡是生物活性化合物的一个有前景的来源，在癌症治疗、神经保护和氧化应激管理方面具有潜在应用。该研究还强调了环境因素对植物化学成分和生物活性的影响，有必要进一步研究其治疗用途的药理潜力。
