Hossain Md Sifat, Patwary Md Abdul Majed, Rupa Sharmin Akther, Kazi Mohsin, Kumar Ashok, Alghamdi Khalid M, Matin Mohammed Mahbubul, Rahman Md Rezaur
Department of Pharmacy, Comilla University, Cumilla-3506, Bangladesh.
Institute for Biomedical Science, Georgia State University, Atlanta, Georgia.
J Cancer. 2025 Apr 28;16(8):2492-2502. doi: 10.7150/jca.94492. eCollection 2025.
has traditionally been applied to treat heart disease, chronic weight loss, improved digestion, memory loss, convulsion and epilepsy, psychological problems, and neurologic pains. Prior studies have already elucidated the potential therapeutic effects of C. religiosa. However, in this work, the bark extract of was studied systematically to investigate its antioxidant, anticholinesterase, and antiproliferative activities, focusing on potential applications in treating Alzheimer's disease (AD) and cancer. The dried coarse powder of bark was cold-extracted in methanol and evaporated to dryness. It was then successively fractionated into petroleum ether (PEF), dichloromethane (DMF), and ethyl acetate (EEF) fractions. The Folin-Ciocalteu reagent and AlCl approaches were utilized to evaluate the total phenol and flavonoid contents, respectively, and the DPPH (2,2-diphenyl-1-picrylhydrazyl) and phosphomolybdenum assays were employed to determine the antioxidant activity of each fraction. The DMF was tested against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) by employing the Ellman method, while all fractions were tested for antiproliferative activity against HepG2 and A549 cell lines by MTT assay. The DMF displayed the highest phenolic content (124.8 ± 17.5 mg gallic acid equivalent/g of dry extract) and flavonoid content (211.1 ± 4.8 mg quercetin equivalent/g of dry extract). In the phosphomolybdenum assay, its antioxidant IC50 value was 25 ± 1 µg/mL. Additionally, the DMF fraction exhibited significant inhibition activity against AChE and BChE, with IC50 values of 455 ± 1 and 450 ± 1 μg/mL, respectively. In terms of anti-proliferative activity, the DMF exhibited an IC50 value of 29.2 µM against the HepG2 cell line, while the EAF showed IC50 values of 24.7 µM in the A549 cell line. The potent activity of as an antioxidant, along with its significant inhibition of AChE and BChE, positions it as a promising candidate for Alzheimer's disease treatment. Furthermore, its robust inhibition of human liver and lung cancer cells suggests its potential as a therapeutic agent against cancer.
传统上一直被用于治疗心脏病、慢性体重减轻、改善消化、记忆力减退、惊厥和癫痫、心理问题以及神经疼痛。先前的研究已经阐明了 religiosa 的潜在治疗效果。然而，在这项工作中，对其树皮提取物进行了系统研究，以调查其抗氧化、抗胆碱酯酶和抗增殖活性，重点关注在治疗阿尔茨海默病（AD）和癌症方面的潜在应用。将其树皮的干燥粗粉用甲醇冷提取并蒸发至干。然后依次将其分离成石油醚（PEF）、二氯甲烷（DMF）和乙酸乙酯（EEF）馏分。分别采用福林-西奥尔特试剂法和 AlCl 法评估总酚和黄酮含量，并采用 DPPH（2,2-二苯基-1-苦基肼）和磷钼酸法测定各馏分的抗氧化活性。采用 Ellman 法检测 DMF 对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的抑制作用，同时采用 MTT 法检测所有馏分对 HepG2 和 A549 细胞系的抗增殖活性。DMF 的酚类含量最高（124.8±17.5 毫克没食子酸当量/克干提取物），黄酮含量最高（211.1±4.8 毫克槲皮素当量/克干提取物）。在磷钼酸法中，其抗氧化 IC50 值为 25±1 微克/毫升。此外，DMF 馏分对 AChE 和 BChE 表现出显著的抑制活性，IC50 值分别为 455±1 和 450±1 微克/毫升。在抗增殖活性方面，DMF 对 HepG2 细胞系的 IC50 值为 29.2 微摩尔，而 EAF 在 A549 细胞系中的 IC50 值为
