Gürgen Ayşenur
Faculty of Engineering and Natural Sciences, Department of Industrial Engineering, Osmaniye Korkut Ata University, Osmaniye, 80000, Turkey.
Sci Rep. 2025 Mar 15;15(1):8981. doi: 10.1038/s41598-025-93499-z.
In this study, the biological activities of the extracts obtained under optimum extraction conditions of the kernel part of Juglans regia L. were determined. Two different methods, Response Surface Method (RSM) and Artificial Neural Network-Genetic Algorithm (ANN-GA) integration, were used for optimization. The antioxidant capacity of the extracts obtained under the extract conditions suggested by the two methods was evaluated by Rel Assay kits, DPPH and FRAP methods. Anticholinesterase activities of the optimized extracts were measured by the action of acetylcholinesterase and butyrylcholinesterase enzymes. Antiproliferative effects of the extracts were tested on A549 lung cancer cell line. Phenolic compounds were analyzed by LC-MS/MS. It was determined that both extracts exhibited strong activities against A549 lung cancer cell line depending on the concentration increase. In addition, it was determined that both extracts exhibited acetyl and butyrylcholinesterase inhibition activity close to galantamine used as a standard. In both extracts, 13 compounds including gallic acid, catechinhyrate, 4-hydroxybenzoic acid, caffeic acid, vanillic acid, syringic acid, 2-hydoxycinamic acid, resveratrol, myricetin, quercetin, kaempferol, protocatechuic acid and 2-hyroxy1,4 naphthaquinone were identified. It was determined that the extract obtained under the conditions predicted by ANN-GA exhibited higher activities in general.
在本研究中，测定了胡桃楸果仁部分在最佳提取条件下获得的提取物的生物活性。采用了两种不同的方法进行优化，即响应面法（RSM）和人工神经网络-遗传算法（ANN-GA）集成。通过Rel检测试剂盒、DPPH和FRAP方法评估了两种方法建议的提取条件下获得的提取物的抗氧化能力。通过乙酰胆碱酯酶和丁酰胆碱酯酶的作用测定了优化提取物的抗胆碱酯酶活性。在A549肺癌细胞系上测试了提取物的抗增殖作用。通过LC-MS/MS分析了酚类化合物。结果表明，两种提取物均对A549肺癌细胞系表现出较强的活性，且活性随浓度增加而增强。此外，还确定两种提取物均表现出接近用作标准的加兰他敏的乙酰胆碱酯酶和丁酰胆碱酯酶抑制活性。在两种提取物中，鉴定出了13种化合物，包括没食子酸、儿茶素、4-羟基苯甲酸、咖啡酸、香草酸、丁香酸、2-羟基肉桂酸、白藜芦醇、杨梅素、槲皮素、山奈酚、原儿茶酸和2-羟基-1,4-萘醌。结果表明，在ANN-GA预测条件下获得的提取物总体上表现出更高的活性。
