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纤维素酶和植物乳杆菌对柠条锦鸡儿青贮性能、微生物群落组成及挥发性成分的影响

Effects of cellulase and Lactiplantibacillus plantarum on silage performance, microbial community composition and volatiles of Caragana korshinskii.

作者信息

Zhang Peiqing, Bai Baochao, Zhou Tianrong, Wang Wei, Zhang Lianyun, Wei Xiaojun, Li Hongbo, Wang Zhijun

机构信息

Grass Industry Collaborative Innovation Research Center, Hulunbuir University, Hulunber, 021000, China.

College of Grassland Science, Inner Mongolia Agricultural University, Hohhot, 010010, China.

出版信息

BMC Microbiol. 2025 Jul 2;25(1):397. doi: 10.1186/s12866-025-04116-y.

Abstract

C as a geogenic non-conventional forage is underutilized due to high lignocellulose and the presence of specific odors. The aim of this study was to investigate the effects of no additives (CK), cellulase (CE) and (LAB) on C silage quality, microbial community structure and volatiles. The results showed that both cellulase and were able to enhance fermentation quality by lowering the pH value and increasing the lactic acid (LA) content of C silage. In addition, cellulase was more effective in decreasing neutral detergent fiber (NDF) content and acid detergent fiber (ADF) content, and increasing water-soluble carbohydrate (WSC) content (<0.05). Microbial community analysis showed that the addition of cellulase significantly reduced the microbial diversity of C silage and simplified the flora structure (<0.05), with the relative abundance of the dominant bacterium accounting for 82.78%, while suppressing undesirable microorganisms such as and . Analysis of volatile compounds showed that terpenes (54.35%) and alcohols (27.49%) were the main volatile organic compounds in fresh samples of C, and the addition of LAB was able to reduce the content of terpene volatiles. Furthermore, silage treatment was able to improve the palatability of C by eliminating irritating odors such as 2-Methylnaphthalene and Naphthalene. These results suggest that silage can improve the odor of C and that cellulase is more beneficial as a silage additive to improve silage quality.

摘要

C作为一种地质成因的非常规饲料,由于其木质纤维素含量高且存在特定气味,未得到充分利用。本研究的目的是探讨不添加任何物质(CK)、纤维素酶(CE)和乳酸菌(LAB)对C青贮饲料品质、微生物群落结构和挥发性物质的影响。结果表明,纤维素酶和乳酸菌均能通过降低pH值和提高C青贮饲料的乳酸(LA)含量来提高发酵品质。此外,纤维素酶在降低中性洗涤纤维(NDF)含量和酸性洗涤纤维(ADF)含量以及增加水溶性碳水化合物(WSC)含量方面更有效(P<0.05)。微生物群落分析表明,添加纤维素酶显著降低了C青贮饲料的微生物多样性并简化了菌群结构(P<0.05),优势细菌乳酸菌的相对丰度占82.78%,同时抑制了诸如肠杆菌属和芽孢杆菌属等不良微生物。挥发性化合物分析表明,萜类化合物(54.35%)和醇类(27.49%)是C新鲜样品中的主要挥发性有机化合物,添加乳酸菌能够降低萜类挥发性物质的含量。此外,青贮处理能够通过消除2-甲基萘和萘等刺激性气味来提高C的适口性。这些结果表明,青贮可以改善C的气味,并且纤维素酶作为青贮添加剂对提高青贮品质更有益。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c79/12219926/167ad672cf57/12866_2025_4116_Fig1_HTML.jpg

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