Biofuels Institute, Jiangsu University, Zhenjiang 212013, China; School of Environment & Safety Engineering, Jiangsu University, Zhenjiang 212013, China; Department of Zoology & Environmental Biology, University of Nigeria, Nsukka 410001, Nigeria.
Biofuels Institute, Jiangsu University, Zhenjiang 212013, China; School of Environment & Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Microbiol Res. 2023 Jan;266:127212. doi: 10.1016/j.micres.2022.127212. Epub 2022 Sep 30.
Ensiling is a microbial-driven process used to preserve fresh forage in bio-refinery and animal production. The biochemical changes that ensue during ensiling have aided the search for new silage additives, emphasizing the potential of certain microbial strains that are more efficient in biopreservation. Lactic acid bacteria (LAB) species are widely recognized for their varied application as additives in the fermentation of crops or forage biomasses during ensiling. However, inconsistency in silage quality in recent times could be interpreted by the lack of information on gene expression and molecular mechanisms of microbiota involved in silage production. Modern research has focused on unraveling nutrient-rich animal feed with improved LAB inoculants. Therefore, this review elucidates the role of LAB inoculants in silage production as well as the modern biotechnology approaches, including metabolomics, proteomics, metagenomics, genomics, transcriptomics, and genetic manipulation, which are powerful tools for identifying, improving, and developing high-performance LAB strains. In addition, the review highlighted the trends and future perspectives of LAB development for silage improvement, pertinent for animal feed breakthroughs in sustainable agriculture.
青贮是一种微生物驱动的过程，用于在生物精炼厂和动物生产中保存新鲜饲料。青贮过程中发生的生化变化有助于寻找新的青贮添加剂，强调了某些微生物菌株在生物保鲜方面更有效率的潜力。乳酸菌（LAB）种类因其在发酵作物或青贮饲料生物量方面的各种应用而被广泛认可。然而，最近青贮质量的不一致性可能是由于缺乏有关参与青贮生产的微生物群落的基因表达和分子机制的信息。现代研究的重点是利用富含营养的动物饲料来改善 LAB 接种物。因此，本综述阐述了 LAB 接种物在青贮生产中的作用，以及现代生物技术方法，包括代谢组学、蛋白质组学、宏基因组学、基因组学、转录组学和遗传操作，这些方法是识别、改进和开发高性能 LAB 菌株的有力工具。此外，该综述还强调了 LAB 开发用于青贮改良的趋势和未来展望，这对可持续农业中的动物饲料突破至关重要。
