Patanita M, Campos M D, Albuquerque A, Ribeiro J A, Pimentel D, Ramiro R S, Monteiro T, Basaloco M, Santos F, Castro I, Félix M R
MED - Mediterranean Institute for Agriculture, Environment and Development & CHANGE - Global Change and Sustainability Institute, Institute for Advanced Studies and Research, Universidade de Évora, Pólo da Mitra, Ap. 94, Évora, 7006-554, Portugal.
MED - Mediterranean Institute for Agriculture, Environment and Development & CHANGE - Global Change and Sustainability Institute, Departamento de Fitotecnia, Escola de Ciências e Tecnologia, Universidade de Évora, Pólo da Mitra, Ap. 94, Évora, 7006-554, Portugal.
BMC Plant Biol. 2025 Jul 2;25(1):811. doi: 10.1186/s12870-025-06763-9.
Grapevine trunk diseases (GTDs) are a disease complex that pose a serious challenge to vineyard productivity worldwide. Their increasing occurrence, coupled with the absence of effective treatments, turns their incidence one of the biggest obstacles to viticulture. A deeper understanding of grapevine defence mechanisms is essential to develop new strategies for a sustainable disease management.
This study explored the transcriptome profiling of GTDs symptomatic and asymptomatic plants of 'Trincadeira' (a slightly susceptible or tolerant cultivar to GTDs) and 'Alicante Bouschet' (a highly susceptible susceptible or susceptible cultivar to GTDs), under natural field conditions. RNA-seq yielded 1 598 differentially expressed genes (DEGs) when comparing cultivars, and 64 DEGs associated with symptomatology, regardless of the cultivar. Transport was revealed as the main biological process involved, predominantly activated in 'Alicante Bouschet', indicating a possible link between these genes and disease progression. Conversely, the relative tolerance of 'Trincadeira' to this disease complex might be supported by the activation of secondary and hormonal metabolism and the differential expression of a set of defence-related genes, which may act as key factors to limit GTDs infection. An important role of the peroxidase gene PER42 in the inhibition of GTDs symptoms was highlighted in this study.
Our results provide novel insights into grapevine resistance mechanisms to GTDs and highlight candidate genes for improving disease tolerance. To the best of our knowledge, this is the first transcriptomic study of naturally infected grapevines with multiple trunk pathogens under natural field conditions. By modulating the activation or inhibition of key plant response regulators, it may be possible to enhance resistance, offering sustainable and effective strategies for successful management of GTDs.
Not applicable.
葡萄树干病害（GTDs）是一种病害复合体，对全球葡萄园生产力构成严峻挑战。其发病率不断上升，加上缺乏有效的治疗方法，使其成为葡萄栽培的最大障碍之一。深入了解葡萄的防御机制对于制定可持续病害管理新策略至关重要。
本研究在自然田间条件下，探究了‘特林卡岱拉’（对GTDs有轻微易感性或耐受性的品种）和‘阿利坎特布歇’（对GTDs高度易感的品种）的GTDs症状植株和无症状植株的转录组图谱。比较品种时，RNA测序产生了1598个差异表达基因（DEGs），且无论品种如何，有64个DEGs与症状学相关。运输被揭示为主要涉及的生物学过程，主要在‘阿利坎特布歇’中被激活，表明这些基因与疾病进展之间可能存在联系。相反，‘特林卡岱拉’对这种病害复合体的相对耐受性可能得到次生代谢和激素代谢的激活以及一组防御相关基因的差异表达的支持，这些基因可能是限制GTDs感染的关键因素。本研究强调了过氧化物酶基因PER42在抑制GTDs症状方面的重要作用。
我们的结果为葡萄对GTDs的抗性机制提供了新见解，并突出了用于提高抗病性的候选基因。据我们所知，这是在自然田间条件下对多种树干病原体自然感染的葡萄进行的首次转录组研究。通过调节关键植物反应调节因子的激活或抑制，可以增强抗性，为成功管理GTDs提供可持续且有效的策略。
不适用。
