Engel D A, Samanta H, Brawner M E, Lengyel P
Virology. 1985 Apr 30;142(2):389-97. doi: 10.1016/0042-6822(85)90346-0.
The exposure of cells to interferons enhances the accumulation of particular mRNAs and of the corresponding proteins. A cDNA clone (clone 202) complementary to an mRNA (202 mRNA) whose level is enhanced over 12-fold in mouse Ehrlich ascites tumor cells upon exposure to beta-interferon for 10 hr has previously been isolated. The level of this mRNA was also increased in other beta-interferon-responsive mouse cell lines (i.e., L929, L1210S) but not in a line (L1210R) which is not responsive to beta-interferon. The extent of induction in Ehrlich ascites tumor cells depended on the beta-interferon concentration and reached its maximal level between 300 and 1000 units of interferon/ml. Nuclei isolated from Ehrlich ascites tumor cells which had been exposed to beta-interferon produced in vitro more 202 specific RNA than nuclei from control Ehrlich ascites tumor cells: an increase in this production was detectable 2 hr after beginning the exposure of the cells to 1000 units/ml of beta-interferon and the increase reached its maximal level, around 18-fold, after 18 hr exposure. Much, if not all of this increase, appeared to be due to an increase in the rate of synthesis of the RNA and not to a decrease in its rate of turnover. The 202 mRNA was translated in a reticulocyte lysate into a 56,000-Da protein.
细胞暴露于干扰素会增强特定mRNA及相应蛋白质的积累。之前已分离出一个与一种mRNA（202 mRNA）互补的cDNA克隆（克隆202），在小鼠艾氏腹水瘤细胞中，该mRNA的水平在暴露于β-干扰素10小时后提高了12倍以上。在其他对β-干扰素敏感的小鼠细胞系（如L929、L1210S）中，这种mRNA的水平也有所增加，但在对β-干扰素不敏感的细胞系（L1210R）中则没有增加。艾氏腹水瘤细胞中的诱导程度取决于β-干扰素的浓度，在干扰素浓度为300至1000单位/毫升时达到最高水平。从暴露于β-干扰素的艾氏腹水瘤细胞中分离出的细胞核，在体外产生的202特异性RNA比对照艾氏腹水瘤细胞的细胞核更多：在细胞开始暴露于1000单位/毫升的β-干扰素2小时后，就可检测到这种产量的增加，在暴露18小时后，增加达到最高水平，约为18倍。这种增加，如果不是全部的话，很大程度上似乎是由于RNA合成速率的增加，而不是其周转速率的降低。202 mRNA在网织红细胞裂解物中被翻译成一种56,000道尔顿的蛋白质。
