Zhang Yaling, Chen Xiuping, Su Wenbing, Deng Chaojun, Jiang Jimou, Zheng Shaoquan
Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fujian, Fuzhou, China.
Front Plant Sci. 2025 Jun 18;16:1615281. doi: 10.3389/fpls.2025.1615281. eCollection 2025.
Loquat ( Lindl.) is a subtropical evergreen tree native to China. Generally, the pigments accumulated in the fruits of cultivated loquats are carotenoids rather than anthocyanins. In this study, we showed that the peel of 'Shanpaisanhao' loquats can accumulate anthocyanins and turn red. We further investigated the mechanisms underlying anthocyanin accumulation in the red-pigmented peels of 'Shanpaisanhao' loquats. RNA-seq analysis demonstrated that anthocyanin accumulation in loquat peel is associated with the upregulation of anthocyanin biosynthetic and transport genes as well as the transcriptional factor Transient overexpression and dual luciferase assays showed that could induce weak anthocyanin accumulation in tobacco leaves when co-expressed with , and activate the promoters of , and . These results provide information for further elucidating the molecular mechanism of anthocyanin accumulation in the peel of SP3H loquat and for breeding of new red-pigmented loquat cultivars.
枇杷（Eriobotrya japonica (Thunb.) Lindl.）是一种原产于中国的亚热带常绿乔木。一般来说，栽培枇杷果实中积累的色素是类胡萝卜素而非花青素。在本研究中，我们发现‘山枇杷三号’枇杷的果皮能够积累花青素并变红。我们进一步研究了‘山枇杷三号’枇杷红色果皮中花青素积累的潜在机制。RNA测序分析表明，枇杷果皮中花青素的积累与花青素生物合成和转运基因以及转录因子的上调有关。瞬时过表达和双荧光素酶分析表明，与 共表达时， 可在烟草叶片中诱导较弱的花青素积累，并激活 、 和 的启动子。这些结果为进一步阐明SP3H枇杷果皮中花青素积累的分子机制以及培育新的红色枇杷品种提供了信息。
