Singh Tripti, Shafi Zaryab, Singh Rahul, Bisht Bhawna, Yadav Krishna Kumar, Algethami Jari S, Albakri Ghadah Shukri, Alreshidi Maha Awjan
Department of Biosciences, Integral University, Lucknow, Uttar Pradesh 226026, India.
Department of Bioengineering, Integral University, Lucknow, Uttar Pradesh 226026, India.
Food Chem X. 2025 Jun 17;29:102672. doi: 10.1016/j.fochx.2025.102672. eCollection 2025 Jul.
This study aimed to enhance the phytochemical yield from L. leaves by optimizing microwave-assisted extraction (MAE) parameters using response surface methodology (RSM). The optimized conditions were found to be 239.6 W (microwave power), 1.58 min (extraction time), and 1:22 (solid-to-solvent ratio). Under these conditions, the extract yield was 8.92 %, with a total phenolic content (TPC) of 77.98 mg GAE/g, total flavonoid content (TFC) of 38.99 mg QUE/g, antioxidant activity of 62.95 %, and chlorophyll content of 42.02 mg/mL. Gas chromatography-mass spectrometry (GC-MS) analysis identified key bioactive compounds, including phytol and neophytadiene. Structural analysis by scanning electron microscopy (SEM) revealed substantial cellular disruption, confirming efficient extraction. Furthermore, the extract demonstrated strong antibacterial activity against , , , and . These findings underscore the effectiveness of optimized MAE in extracting potent phytochemicals from betel leaves, reinforcing its potential in natural product development and therapeutic applications.
本研究旨在通过响应面法（RSM）优化微波辅助萃取（MAE）参数，提高罗勒叶的植物化学物质产量。优化后的条件为239.6瓦（微波功率）、1.58分钟（萃取时间）和1:22（固液比）。在这些条件下，提取物产率为8.92%，总酚含量（TPC）为77.98毫克没食子酸当量/克, 总黄酮含量（TFC）为38.99毫克槲皮素当量/克，抗氧化活性为62.95%，叶绿素含量为42.02毫克/毫升。气相色谱-质谱联用（GC-MS）分析鉴定出关键生物活性化合物，包括叶绿醇和新植二烯。扫描电子显微镜（SEM）进行的结构分析显示细胞大量破裂，证实了萃取效率。此外，提取物对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌和铜绿假单胞菌表现出强大的抗菌活性。这些发现强调了优化后的微波辅助萃取在从罗勒叶中提取有效植物化学物质方面的有效性，增强了其在天然产物开发和治疗应用中的潜力。
