Iwahashi Yasuyuki, Nishikage Seiji, Hosokawa Yusei, Yamada Tomoko, Nakagawa Yasushi, Hirota Yushi, Sakaguchi Kazuhiko, Nishimoto Yuki, Nakajima Shinsuke, Kitahama Seiichi, Yamamoto Yasuyoshi, Mukai Tomoichiro, Ogawa Wataru, Tamori Yoshikazu
Division of Diabetes and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-Cho, Chuo-Ku, Kobe, 650-0017 Japan.
Department of Nutrition, Kobe University Hospital, 7-5-2 Kusunoki-Cho, Chuo-Ku, Kobe, 650-0017 Japan.
Diabetol Int. 2025 Apr 8;16(3):538-545. doi: 10.1007/s13340-025-00806-0. eCollection 2025 Jul.
The cell death-inducing DFF45-like effector (CIDE) family comprises CIDEA, CIDEB, and CIDEC (fat-specific protein of 27 kDa), all of which are lipid droplet-associated proteins and contribute to fat storage and energy homeostasis. However, the relative impacts of these isoforms on fat storage in adipose tissue and obesity in humans have been unclear. We here examined the expression of CIDE family genes in visceral adipose tissue (VAT) of obese individuals who underwent laparoscopic sleeve gastrectomy and explored its relation to adiposity-related parameters. RNA-sequencing analysis revealed that and were highly expressed in VAT, whereas was expressed at a substantially lower level. and expression levels were positively correlated with body fat mass and subcutaneous adipose tissue (SAT) area, whereas expression was negatively correlated with these markers. In addition, and expression levels showed positive and negative correlations, respectively, with BMI. Multivariable regression analysis showed that only expression was significantly associated with body fat mass. Stratification of the subjects according to tertiles of expression revealed that BMI, body fat mass, and SAT area were significantly greater in the highest expression group than in the other two groups. Our findings thus suggest that, among CIDE isoforms, CIDEC is the most closely associated with fat storage in human adipose tissue.
The online version contains supplementary material available at 10.1007/s13340-025-00806-0.
细胞死亡诱导DFF45样效应蛋白（CIDE）家族包括CIDEA、CIDEB和CIDEC（27 kDa脂肪特异性蛋白），它们均为脂滴相关蛋白，有助于脂肪储存和能量稳态。然而，这些异构体对人体脂肪组织中脂肪储存和肥胖的相对影响尚不清楚。我们在此研究了接受腹腔镜袖状胃切除术的肥胖个体内脏脂肪组织（VAT）中CIDE家族基因的表达，并探讨了其与肥胖相关参数的关系。RNA测序分析显示，[此处原文缺失具体基因名]和[此处原文缺失具体基因名]在VAT中高表达，而[此处原文缺失具体基因名]表达水平显著较低。[此处原文缺失具体基因名]和[此处原文缺失具体基因名]的表达水平与体脂量和皮下脂肪组织（SAT）面积呈正相关，而[此处原文缺失具体基因名]的表达与这些标志物呈负相关。此外，[此处原文缺失具体基因名]和[此处原文缺失具体基因名]的表达水平分别与BMI呈正相关和负相关。多变量回归分析显示，只有[此处原文缺失具体基因名]的表达与体脂量显著相关。根据[此处原文缺失具体基因名]表达的三分位数对受试者进行分层显示，最高[此处原文缺失具体基因名]表达组的BMI、体脂量和SAT面积显著高于其他两组。因此，我们的研究结果表明，在CIDE异构体中，CIDEC与人类脂肪组织中的脂肪储存关系最为密切。
在线版本包含可在10.1007/s13340-025-00806-0获取的补充材料。
