Essa Mohamed Osman Abdalrahem, Cheng Cheng, Li Jun, Han Xiao, Wei Zhong Kang, Abdelhadi Layla Ahmed Mohammed, Hassan Huda Ahmed, Adam Saber Y, Husien Hosameldeen Mohamed, Saleh Ahmed A, Cheng Darong
College of Veterinary Medicine, Yangzhou University, Yangzhou, China.
College of Veterinary Medicine, Albutana University, Rufaa, Sudan.
Front Vet Sci. 2025 Jun 18;12:1604638. doi: 10.3389/fvets.2025.1604638. eCollection 2025.
Probiotic interventions in young livestock are gaining attention for their potential health benefits.
This study involved 15 weaned goat kids (2-3 months old; 10-15 kg body weight), including 10 healthy kids and 5 diarrheic kids. The kids were divided into three groups: Healthy Control (H, no treatment), Probiotic-Treated Healthy (T), and Diarrheic + Probiotic-Treated (D). All kids were maintained under standardized environmental conditions and fed a controlled diet (60% corn, 15% pea skin, 15% silage, 5% hay and 1% vitamin-mineral additives). Probiotic bacteria and were administered via oral gavage at a concentration of (1 × 10) CFU/mL for five consecutive days. Fecal samples were collected for sequencing of the bacterial 16S rRNA gene to analyze microbial composition.
Healthy groups exhibited significantly greater species richness and diversity compared to the diarrheal group (p < 0.01). The predominant phyla identified were , , and .Increased levels of , , , and were noted in healthy treated kids, while UG_001 and decreased.
The findings highlight significant gut microbiota differences between healthy and diarrheal kids, suggesting that modifications in gut microbiota composition could alleviate diarrhea, contributing to preventive and therapeutic strategies for this condition.
益生菌干预幼畜因其潜在的健康益处而受到关注。
本研究涉及15只断奶山羊羔（2 - 3个月大；体重10 - 15千克），其中包括10只健康羔羊和5只腹泻羔羊。这些羔羊被分为三组：健康对照组（H，不治疗）、益生菌处理的健康组（T）和腹泻 + 益生菌处理组（D）。所有羔羊均饲养在标准化环境条件下，并给予控制饮食（60%玉米、15%豆皮、15%青贮饲料、5%干草和1%维生素 - 矿物质添加剂）。益生菌细菌以（1×10）CFU/mL的浓度通过口服灌胃连续给药五天。收集粪便样本进行细菌16S rRNA基因测序以分析微生物组成。
与腹泻组相比，健康组的物种丰富度和多样性显著更高（p < 0.01）。鉴定出的主要门为 、 和 。在接受益生菌处理的健康羔羊中， 、 、 和 的水平升高，而UG_001和 降低。
研究结果突出了健康羔羊和腹泻羔羊之间肠道微生物群的显著差异，表明肠道微生物群组成的改变可能缓解腹泻，有助于制定针对这种情况的预防和治疗策略。
