Vlasov G S, Torchilin V P, Gremiakova T A, Likhoded V G, Korosteleva M D
Zh Mikrobiol Epidemiol Immunobiol. 1985 Aug(8):87-91.
For the first time the study of the indicator system consisting of sensitized liposomes with NaF incorporated as a marker and a fluorine-selective electrode has been made and, as a result, the possibility of the potentiometric determination of the immune lysis of liposomes in the presence of complement and specific antibodies has been demonstrated. The dissolution of the lipid components (Re-chemotype glycolipid and lipid A) in the bilayer matrix obviates the necessity for converting lipid antigens into the water-soluble state in the process of serological tests. As compared with other methods, the liposomal potentiometric method for the determination of Re-chemotype glycolipid and lipid A is highly sensitive (20-40 ng/ml), rapid, technically easy to perform, cheap and does not require large volumes of samples. The disadvantages of this analytical system are the instability of liposomes and the diffusion of fluorine ions from the internal aqueous phase of vesicules. For this reason the immunoassay can be made only within 12 hours after the preparation of sensitized liposomes incorporating the marker.
首次对由掺入氟化钠作为标记物的致敏脂质体和氟选择性电极组成的指标体系进行了研究，结果表明，在补体和特异性抗体存在的情况下，用电位滴定法测定脂质体免疫裂解的可能性得到了证实。双层基质中脂质成分（Re-化学型糖脂和脂质A）的溶解消除了在血清学检测过程中将脂质抗原转化为水溶性状态的必要性。与其他方法相比，用于测定Re-化学型糖脂和脂质A的脂质体电位滴定法灵敏度高（20-40 ng/ml）、速度快、技术操作简便、成本低且不需要大量样品。该分析系统的缺点是脂质体不稳定以及氟离子从小泡内部水相扩散。因此，免疫测定只能在制备掺入标记物的致敏脂质体后12小时内进行。
