Kotova T A, Pogorel'skaia L V, Il'inskiĭ Iu A, Likhoded V G, Poverennyĭ A M
Zh Mikrobiol Epidemiol Immunobiol. 1982 Sep(9):103-7.
The ability of blood sera obtained from healthy persons and typhoid patients to induce agglutination of red blood cells sensitized with chemotype Re glycolipid has been studied. The blood sera of healthy persons have been shown to possess low endotoxin-binding activity (the reciprocal titer amounts to 21.6 +/- 3.7). In typhoid patients with a severe clinical course of the disease the titers are slightly elevated (49.8 +/- 12.8), in patients with a moderate course a sharp elevation of the titers is observed (240.3 +/-32.8), and in chronic carriers the titers are at a medium level (119 +/- 24.7). The highest titers (288 +/- 72.2) were observed during the first 2 weeks in a moderately severe course of the disease, and by the end of the disease these titers dropped (143.1 +/0 27.4). The endotoxin-binding activity of the sera was inhibited by glycolipid Re and dextran sulfate. The activity of the sera decreased after treatment with 2-mercaptoethanol. The conclusion on a protective role played by antibodies to glycolipid Re and by proteins binding glycolipid and other polyanions has been made on the basis of the data obtained in this study.
研究了从健康人和伤寒患者获得的血清诱导化学型Re糖脂致敏红细胞凝集的能力。已证明健康人的血清具有低内毒素结合活性（倒数滴度为21.6±3.7）。在疾病临床过程严重的伤寒患者中，滴度略有升高（49.8±12.8），在病情中等的患者中观察到滴度急剧升高（240.3±32.8），而在慢性携带者中滴度处于中等水平（119±24.7）。在疾病中度严重过程的前2周观察到最高滴度（288±72.2），到疾病末期这些滴度下降（143.1±27.4）。血清的内毒素结合活性被Re糖脂和硫酸葡聚糖抑制。用2-巯基乙醇处理后血清活性降低。基于本研究获得的数据，得出了抗Re糖脂抗体以及结合糖脂和其他多阴离子的蛋白质发挥保护作用的结论。
