Tong Jiabei, Alzahrani Badr, Subbiah Suresh Kumar, Rampal Sanjiv, Ramasamy Rajesh, Ma Zhijie, Wu Xiaoyun, Mok Pooi Ling
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.
Department of Sports Medicine, The People's Hospital of Wu Hai Inner Mongolia, Wuhai, China.
Sci Rep. 2025 Jul 3;15(1):23748. doi: 10.1038/s41598-025-07642-x.
Meniscus-derived stem cells (MeSCs) hold great promise for cytotherapy of the meniscus. Large-scale cell expansion in vitro is usually accompanied by decreased proliferation and migration and increased senescence, leading to a decrease in treatment efficiency. Therefore, the present study aimed to compare the effects of different concentrations of glucose on the proliferation, migration, senescence and protein expression of MeSCs. In this study, human MeSCs were cultured in two types of expansion media: low-glucose (LG) DMEM (5.5 mM glucose) and high-glucose (HG) DMEM (25 mM glucose). At specific passage number, the proliferation rate was evaluated by cell counting, the migration rate was evaluated by a scratch-wound assay, cell senescence was evaluated by β-GAL assays, and protein expression/phosphorylation was evaluated by ELISA. The results showed that a lower concentration of glucose promoted proliferation and migration, reduced cell senescence, and activated PI3K/Akt-related signaling pathways. This study provides a preliminary basis for the use of this medium composition for MeSC expansion.
半月板来源的干细胞（MeSCs）在半月板细胞治疗方面具有巨大潜力。体外大规模细胞扩增通常伴随着增殖和迁移能力下降以及衰老增加，导致治疗效率降低。因此，本研究旨在比较不同浓度葡萄糖对MeSCs增殖、迁移、衰老和蛋白表达的影响。在本研究中，人MeSCs在两种类型的扩增培养基中培养：低糖（LG）DMEM（5.5 mM葡萄糖）和高糖（HG）DMEM（25 mM葡萄糖）。在特定传代次数时，通过细胞计数评估增殖率，通过划痕试验评估迁移率，通过β-半乳糖苷酶检测评估细胞衰老，通过酶联免疫吸附测定（ELISA）评估蛋白表达/磷酸化。结果表明，较低浓度的葡萄糖促进增殖和迁移，减少细胞衰老，并激活PI3K/Akt相关信号通路。本研究为使用这种培养基成分进行MeSC扩增提供了初步依据。
