Oh Moon Sik, Hong Heesun, Lee Ok Joo, Yi Su Hyeon, Park Hae Sang, Lee Jae-Jun, Park Chan Hum, Im Sun-Wha
Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 24252, Republic of Korea.
Department of Biochemistry and Molecular Biology, Kangwon National University School of Medicine, Chuncheon 24341, Republic of Korea.
Genes (Basel). 2024 Dec 19;15(12):1626. doi: 10.3390/genes15121626.
BACKGROUND/OBJECTIVES: Tonsil-derived mesenchymal stem cells (TMSCs) are in the limelight in regenerative medicine due to their high proliferation and differentiation potential. It is important to conduct studies to determine the optimal conditions for achieving the maximum yield while maintaining the optimal differentiation capacity of TMSCs.
This study explores the impact of serial subculture on TMSCs by analyzing gene expression at passages 2, 4, 6, and 8. For each culture passage, genes with significant differences in RNA expression from previous passages were selected and their characteristics were observed performing enrichment analysis including KEGG (Kyoto Encyclopedia of Genes and Genomes) and Reactome pathway.
At each passage, a "cell cycle" term was ranked high with statistical significance in the KEGG and Reactome pathway. Cell cycle gene expression, including Cyclin-dependent kinases (CDKs) and cyclins, increased until passage 6, then decreased by passage 8. The cell cycle is known to be important not only for proliferation but also for determining whether stem cells maintain pluripotency or differentiate into various lineages.
The results suggest that cell cycle gene expression can guide the timing for differentiation induction, with passage 6 potentially being a critical point for initiating differentiation.
背景/目的：扁桃体来源的间充质干细胞（TMSCs）因其高增殖和分化潜能而在再生医学中备受关注。开展研究以确定在维持TMSCs最佳分化能力的同时实现最大产量的最佳条件非常重要。
本研究通过分析第2、4、6和8代的基因表达来探讨连续传代对TMSCs的影响。对于每一代培养，选择与前一代相比RNA表达有显著差异的基因，并通过进行包括KEGG（京都基因与基因组百科全书）和Reactome通路在内的富集分析来观察其特征。
在每一代，“细胞周期”术语在KEGG和Reactome通路中均具有统计学意义的高排名。包括细胞周期蛋白依赖性激酶（CDKs）和细胞周期蛋白在内的细胞周期基因表达在第6代之前增加，然后在第8代时下降。已知细胞周期不仅对增殖很重要，而且对于确定干细胞是维持多能性还是分化为各种谱系也很重要。
结果表明细胞周期基因表达可以指导分化诱导的时机，第6代可能是启动分化的关键点。
