通过傅里叶变换红外光谱法进行进化枝区分及追踪多中心耳念珠菌（解脂耶氏酵母样念珠菌）暴发中的克隆传播

Clade Distinction and Tracking of Clonal Spread by Fourier-Transform Infrared Spectroscopy in Multicenter Candida (Candidozyma) auris Outbreak.

作者信息

de Melo Camylla C, de Oliveira Halana L N L, Souza Bruna R, Moura Carla V R, Oliveira Rodrigo, Bastos Rafael W, Kemmerich Karoline Kristina, de Almeida-Júnior João N, Colombo Arnaldo Lopes, Spruijtenburg Bram, Meis Jacques F, Le Pape Patrice, Moonen Carolyn G J, de Lima-Neto Reginaldo G

机构信息

Laboratory for Research and Diagnosis of Tropical Diseases, Center for Medical Sciences, Federal University of Pernambuco (UFPE), Recife, Brazil.

Post-Graduation Program in Tropical Medicine, Federal University of Pernambuco (UFPE), Recife, Brazil.

出版信息

Mycoses. 2025 Jul;68(7):e70085. doi: 10.1111/myc.70085.

DOI:10.1111/myc.70085

PMID:40611705

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12232120/

Abstract

BACKGROUND

Candida (Candidozyma) auris has distinct genetic clades. Clade distinction is relevant for infection control and epidemiological purposes. State-of-the-art typing methodologies for clade distinction are based on genomic approaches, such as short tandem repeat (STR) analysis and whole-genome sequencing (WGS). However, they are time-consuming and expensive. Fourier transform infrared spectroscopy (FT-IR) is an alternative tool for strain typing based on their unique biochemical spectral profiles.

OBJECTIVES

To apply FT-IR to differentiate C. auris clades and evaluate epidemiological relationships based on biochemical data among isolates from a multicenter C. auris outbreak in the state of Pernambuco, northeastern Brazil.

METHODS

Sixty-nine C. auris strains from clades I, II, III, and IV were analysed. Fifty-nine were clade IV strains obtained from three outbreaks that took place in Pernambuco state, northeastern Brazil. An adjusted FT-IR spectroscopy protocol was applied to obtain carbohydrates and lipid fingerprints. Short Tandem Repeat (STR) analysis was used in order to validate the spectroscopy approach.

RESULTS

The adjusted preparation protocol for FT-IR analysis improved the spectral quality by 31.42% compared to standard protocol. FT-IR allowed us to discriminate C. auris clades I to IV. Moreover, important similarities were observed in C. auris clade IV strains obtained from two of the three hospitals, based on polysaccharides (1300-800 cm) plus lipids (3000-2800 cm and 1500-1400 cm) spectra. STR confirmed the similarity results obtained by FT-IR, clustering the strains from two different hospitals.

CONCLUSIONS

The IR Biotyper is fast, easy-to-use, and a promising alternative for moderate-to-high-complexity laboratories to differentiate C. auris clades. Furthermore, this technique has the potential for isolate-level source tracking, which could be valuable for monitoring transmission routes in clinical settings.

摘要

背景

耳念珠菌（念珠地丝菌）具有不同的遗传分支。分支区分对于感染控制和流行病学目的具有重要意义。用于分支区分的先进分型方法基于基因组方法，如短串联重复序列（STR）分析和全基因组测序（WGS）。然而，这些方法既耗时又昂贵。傅里叶变换红外光谱（FT-IR）是一种基于菌株独特生化光谱特征进行菌株分型的替代工具。

目的

应用傅里叶变换红外光谱区分耳念珠菌分支，并根据巴西东北部伯南布哥州多中心耳念珠菌暴发分离株的生化数据评估流行病学关系。

方法

分析了来自I、II、III和IV分支的69株耳念珠菌菌株。其中59株是从巴西东北部伯南布哥州发生的三次暴发中获得的IV分支菌株。应用经过调整的傅里叶变换红外光谱协议获取碳水化合物和脂质指纹图谱。使用短串联重复序列（STR）分析来验证光谱学方法。

结果

与标准协议相比，用于傅里叶变换红外光谱分析的调整后制备协议使光谱质量提高了31.42%。傅里叶变换红外光谱使我们能够区分耳念珠菌的I至IV分支。此外，基于多糖（1300 - 800 cm）加脂质（3000 - 2800 cm和1500 - 1400 cm）光谱，在从三家医院中的两家获得的耳念珠菌IV分支菌株中观察到重要的相似性。STR证实了傅里叶变换红外光谱获得的相似性结果，将来自两家不同医院的菌株聚类在一起。