Donaldson J C, Stoop D R, Williams S A
Chest. 1977 Jul;72(1):10-2. doi: 10.1378/chest.72.1.10.
In a double-blind study the fiberoptic bronchoscope was contaminated with five pathogenic organisms, each at a concentration of 10(9) organisms per milliliter. The shaft and inner channel of the bronchoscope were cleansed with five antiseptic regimens. Each regimen included equal 120-ml aliquots of one, two, or three solutions, respectively. The five different regimens were as follows: (1) physiologic saline solution; (2) 70 percent solution of isopropyl alcohol, followed by physiologic saline solution; (3) alkaline glutaraldehyde, followed by 70 percent solution of isoproply alcohol, followed by physiologic saline solution; (4) benzalkonium chloride, followed by 70 percent isopropyl alcohol, followed by physiologic saline solution; and (5) povidone-iodine solution, followed by 70 percent solution of isopropyl alcohol, followed by physiologic saline solution. The four regimens involving solutions other than saline solution alone were effective in reducing the count of residual bacterial colonies to 10(4) colonies per milliliter or less.
在一项双盲研究中，将纤维支气管镜用五种致病生物体污染，每种生物体的浓度均为每毫升10⁹个生物体。用五种防腐方案对支气管镜的镜身和内部通道进行清洁。每种方案分别包含等量的120毫升一份、两份或三份溶液。五种不同的方案如下：(1) 生理盐水溶液；(2) 70%异丙醇溶液，随后用生理盐水溶液；(3) 碱性戊二醛，随后用70%异丙醇溶液，再用生理盐水溶液；(4) 苯扎氯铵，随后用70%异丙醇，再用生理盐水溶液；以及(5) 聚维酮碘溶液，随后用70%异丙醇溶液，再用生理盐水溶液。除仅使用盐溶液的方案外，涉及其他溶液的四种方案均能有效将残留细菌菌落数减少至每毫升10⁴个菌落或更少。
