Lu Xueqin, Gao Yijing, Liu Xinyu, Sun Yibo, Zhen Guangyin
School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, PR China; Institute of Eco-Chongming (IEC), 3663 N. Zhongshan Rd., Shanghai 200062, PR China; Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, Shanghai 200241, PR China.
School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, PR China.
Bioresour Technol. 2025 Nov;435:132934. doi: 10.1016/j.biortech.2025.132934. Epub 2025 Jul 2.
Ammonia nitrogen (NH-N) and sulfate (SO) removal by Anaerobic ammonium oxidation (Anammox) and sulfate-reducing bacteria (SRB) was studied in dual-chamber microbial electrolysis cells (MECs). Appropriate anode potential stimulation promoted biofilm formation and enhanced extracellular polymeric substances fluorescence, facilitating electron transfer. The highest NH-N removal (81.1 %) was achieved at the anode potential of 0.6 V vs. Ag/AgCl after 50 days, coinciding with the increase in electroactive Candidatus_Brocadia from 1.1 % to 27.4 %. Simultaneously, SO removal reached 77.0 %, supported by cathodic biofilms dominated by SRB (Desulfofustis, Desulfomicrobium, and Desulfatirhabdium). Automated machine learning and principal co-ordinates analysis identified the anode potential as the key factor shaping microbial ecology. The appropriate anode potential (0.4-0.6 V vs. Ag/AgCl) promoted cathodic sulfidogenesis, indirectly enhancing electron flow and supporting Anammox process at the anode. These findings demonstrate that MECs hold great promise for simultaneously enhancing anaerobic ammonia oxidation bacteria and SRB activities, enabling efficient NH-N and SO removal.
在双室微生物电解池（MEC）中研究了厌氧氨氧化（Anammox）和硫酸盐还原菌（SRB）对氨氮（NH-N）和硫酸盐（SO）的去除情况。适当的阳极电位刺激促进了生物膜的形成，并增强了胞外聚合物的荧光，有利于电子转移。50天后，在相对于Ag/AgCl为0.6 V的阳极电位下实现了最高的NH-N去除率（81.1%），同时电活性的“Candidatus_Brocadia”从1.1%增加到27.4%。与此同时，SO去除率达到77.0%，这得益于以SRB（脱硫梭菌属、脱硫微菌属和脱硫嗜盐碱杆菌属）为主的阴极生物膜。自动机器学习和主坐标分析确定阳极电位是塑造微生物生态的关键因素。适当的阳极电位（相对于Ag/AgCl为0.4 - 0.6 V）促进了阴极硫化作用，间接增强了电子流动并支持阳极的厌氧氨氧化过程。这些发现表明，MEC在同时增强厌氧氨氧化细菌和SRB活性、实现高效的NH-N和SO去除方面具有巨大潜力。
