James Jerome Rahul, Jeyapalan Karthigeyan, Natarajan Shanmuganathan
Department of Prosthodontics, Sri Ramachandra Dental College and Hospital, SRIHER, Chennai, India.
J Oral Biol Craniofac Res. 2025 Sep-Oct;15(5):932-937. doi: 10.1016/j.jobcr.2025.06.016. Epub 2025 Jun 22.
To evaluate and compare osteoblastic activity and osseointegration between single-drill and sequential-drill implant site preparation techniques, using osteoprotegerin (OPG) levels as a biomarker.
A randomized controlled trial with a split-mouth design was conducted, involving 12 participants (24 dental implants). The study included two groups: a single-drill group and a sequential-drill group. Gingival crevicular fluid samples were collected at baseline, 15-, 30- and 60-days post-implantation. Osteoprotegerin levels were measured using an enzyme-linked immunosorbent assay (ELISA).
Intragroup analysis showed significant variations in OPG levels over time (p < 0.0001). Intergroup comparisons revealed no significant differences at baseline (p = 0.9563) or at 15 days (p = 0.7617); however, significant differences were detected between groups at 30 days (p = 0.0391) and 60 days (p = 0.0005). The single-drill group exhibited higher initial OPG levels, while the sequential-drill group had elevated OPG levels at 30 and 60 days.
The single-drill technique may provide an early advantage in osteoblastic response, while the sequential-drill technique supports increased osteoblastic activity at later stages. Both techniques effectively support osseointegration, with selection depending on specific clinical requirements.
以骨保护素（OPG）水平作为生物标志物，评估和比较单钻孔与序贯钻孔种植位点预备技术之间的成骨细胞活性和骨整合情况。
采用随机对照、分口设计的试验，纳入12名参与者（24颗牙种植体）。研究分为两组：单钻孔组和序贯钻孔组。在基线、种植后15天、30天和60天采集龈沟液样本。采用酶联免疫吸附测定（ELISA）法测量骨保护素水平。
组内分析显示，OPG水平随时间有显著变化（p < 0.0001）。组间比较显示，基线时（p = 0.9563）和15天时（p = 0.7617）无显著差异；然而，30天时（p = 0.0391）和60天时（p = 0.0005）两组间存在显著差异。单钻孔组初始OPG水平较高，而序贯钻孔组在30天和60天时OPG水平升高。
单钻孔技术可能在成骨细胞反应方面具有早期优势，而序贯钻孔技术在后期可支持更高的成骨细胞活性。两种技术均能有效支持骨整合，具体选择取决于特定的临床需求。
