Brown W J
Health Lab Sci. 1977 Jul;14(3):172-6.
The Modified Rapid Fermentation Test (MRFT) for Neisseria gonorrhoeae has been shown to demonstrate the proper carbohydrate degradation for confirmation in less than 30 minutes. A new approach for using the reagents in the testing procedure has been established, making the MRFT a more practical tool in the laboratory. Instead of mixing reagents at the time of testing, previously prepared, labelled reagents were made in economic quantities and used according to the established procedure. These prepared reagents were stored at -70 C, -20 C, 4 C, and at room temperature (22-23 C). Stability was determined over a 6-month period by using a laboratory strain and fresh clinic strains of N. gonorrhoeae. The use of prepared reagents increases the efficiency of the confirmation approach, reduces the cost per test, and increases the uniformity of the individual tests.
已证明，用于淋病奈瑟菌的改良快速发酵试验（MRFT）能够在不到30分钟的时间内显示出适当的碳水化合物降解情况，以进行确认。在测试过程中使用试剂的新方法已经确立，这使得MRFT在实验室中成为一种更实用的工具。在测试时不再混合试剂，而是以经济的数量制备预先标记好的试剂，并按照既定程序使用。这些制备好的试剂分别储存在-70℃、-20℃、4℃和室温（22 - 23℃）下。通过使用淋病奈瑟菌的实验室菌株和新鲜临床菌株，在6个月的时间内测定了其稳定性。使用制备好的试剂提高了确认方法的效率，降低了每次测试的成本，并提高了各个测试的一致性。
