Remcho T Parks, Clark Robert D E, Martin Alexander J, Kumaran Vaishnavi R, Bhat Nysa, McLahlan Ian, Kolls Jay K
Center for Translational Research in Infection and Inflammation, Tulane University School of Medicine.
Communications Marketing, Tulane University School of Medicine.
J Vis Exp. 2025 Jun 17(220). doi: 10.3791/67676.
Consistent delivery of liquids, including potential therapies, to the lungs is critical for modeling pulmonary disease and pre-clinical therapeutic development. However, current methods used in mice -such as oropharyngeal aspiration by the tongue-pull method- can result in variable delivery. Here, we describe the use of an affordable, mobile phone-linked, lighted otoscope to visualize the glottis and permit direct inoculation into the airway. We detail an optimized staging technique with adequate lighting and positioning aids. How intubation can be reliably conducted using a 20 G flexible catheter with or without a fiberoptic light is also described. This technique greatly reduces variance in intrapulmonary delivery and increases the volume of delivered liquid that reaches the lungs. Due to reduced variance, smaller animal cohort sizes can be used when employing this method instead of oropharyngeal instillation by the tongue-pull method. What follows is a method for effective, rapid, and consistent intubation of mice and delivery to the lungs, including robust confirmation of endotracheal tube placement. With this improved methodology, consistent, safe intubation and replicable, efficient delivery to the lungs are within reach.
将包括潜在治疗药物在内的液体持续输送到肺部，对于模拟肺部疾病和临床前治疗开发至关重要。然而，目前在小鼠中使用的方法，如通过拉舌法进行口咽抽吸，可能会导致输送量不稳定。在此，我们描述了一种使用价格低廉、与手机相连的带光源耳镜来观察声门并直接将药物接种到气道的方法。我们详细介绍了一种具有充足照明和定位辅助工具的优化分期技术。还描述了如何使用带或不带光纤灯的20G柔性导管可靠地进行插管。该技术极大地减少了肺内给药的差异，并增加了到达肺部的给药液体量。由于差异减小，采用该方法时可以使用比拉舌法口咽滴注更小的动物样本量。以下是一种对小鼠进行有效、快速且一致的插管并输送到肺部的方法，包括对气管插管位置的可靠确认。通过这种改进的方法，可以实现一致、安全的插管以及可重复、高效的肺部给药。
