Suppr超能文献

探索水杨酸和茉莉酸甲酯诱导剂对辣椒(Capsicum annuum)生长性能、表观遗传调控、基因转录和次生代谢的影响:一项体外生物学评估。

Exploring effectiveness of salicylic acid and methyl jasmonate elicitors in influencing growth performance, epigenetic regulation, gene transcription, and secondary metabolism in pepper (Capsicum annuum): an in vitro biological assessment.

作者信息

Hasanvand Zeinab, Iranbakhsh Alireza, Saadatmand Sara, Oraghi Ardebili Zahra

机构信息

Institute of Converging sciences and Technologies, SR.C., Islamic Azad University, Tehran, Iran.

Department of Biology, Ga.C., Islamic Azad University, Garmsar, Iran.

出版信息

Protoplasma. 2025 Jul 8. doi: 10.1007/s00709-025-02092-8.

Abstract

Epigenetic responses of in vitro-cultured plants to elicitation with salicylic acid (SA) and methyl jasmonate (MJ) have been little explored. This study addresses how pepper seedlings respond to incorporating SA (0 and 10 µM) and/or MJ (0 and 10 µM) into the culture medium at physiological and molecular levels. The individual application of MJ or SA enhanced root fresh weight by an average of 59.8%. However, the mixed treatment synergistically augmented the root biomass by 2.2-fold. SA was individually more effective than MJ in triggering changes in the DNA methylome profiles. The epigenetic cytosine methylation in the MJ + SA group differed from that of the individual treatments. The individual SA treatment led to severe DNA hypermethylation, which was modified with the MJ application. Moreover, MJ and/or SA-mediated upregulation of the histone deacetylase (HDA) gene also confirmed the epigenetic regulation at the histone modification level. The MJ elicitor showed greater effectiveness in influencing the transcription of the target genes than the SA one. The expression of the MAPK kinase and ethylene-responsive transcription factor (EREB) genes displayed a similar upward trend. Applying pepper seedlings with MJ and SA increased proline concentrations and catalase activities. The simultaneous application of MJ and SA supplements effectively stimulated secondary metabolism, indicated by increased soluble phenols and activity of the phenylalanine ammonia-lyase enzyme. This study presents novel comparative insights into the SA and MJ-mediated epigenetic modifications. These findings can be employed to design in vitro culture systems and implement large-scale projects.

摘要

体外培养植物对水杨酸(SA)和茉莉酸甲酯(MJ)诱导的表观遗传反应鲜有研究。本研究探讨了辣椒幼苗在生理和分子水平上如何响应在培养基中添加SA(0和10 μM)和/或MJ(0和10 μM)。单独施用MJ或SA可使根鲜重平均增加59.8%。然而,混合处理使根生物量协同增加了2.2倍。在触发DNA甲基化组图谱变化方面,SA单独比MJ更有效。MJ + SA组的表观遗传胞嘧啶甲基化与单独处理不同。单独的SA处理导致严重的DNA高甲基化,而MJ处理可对其进行修饰。此外,MJ和/或SA介导的组蛋白脱乙酰酶(HDA)基因上调也证实了在组蛋白修饰水平上的表观遗传调控。MJ诱导剂在影响靶基因转录方面比SA诱导剂更有效。促分裂原活化蛋白激酶激酶和乙烯响应转录因子(EREB)基因的表达呈现相似的上升趋势。用MJ和SA处理辣椒幼苗可提高脯氨酸浓度和过氧化氢酶活性。MJ和SA补充剂的同时施用有效刺激了次生代谢,表现为可溶性酚类增加和苯丙氨酸解氨酶活性增强。本研究提供了关于SA和MJ介导的表观遗传修饰的新颖比较见解。这些发现可用于设计体外培养系统和实施大规模项目。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验