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在多棘海盘车(Asterias amurensis)腕部截肢后的再生过程中转录组的从头组装

De novo assembly of transcriptome during regeneration post-arm amputation in the starfish, Asterias amurensis.

作者信息

Jo Mi Jeong, Go Hye-Jin, Kim Jeong Gyu, Kim Gun-Do

机构信息

Department of Microbiology, Pukyong National University, Busan, 48513, South Korea.

Industry-University Cooperation Foundation, Pukyong National University, Busan, 48513, South Korea.

出版信息

BMC Genom Data. 2025 Jul 8;26(1):45. doi: 10.1186/s12863-025-01340-3.

DOI:10.1186/s12863-025-01340-3
PMID:40624477
Abstract

OBJECTIVES

This study investigates the nerve cord transcriptome of Asterias amurensis to explore its regenerative abilities. By comparing gene expression between a normal group and a group 72 h post-amputation, key genes involved in regeneration were identified. Functional annotation using GO, KEGG, NR, and UniProt databases provided insights into the biological roles of these genes. This research enhances the understanding of A. amurensis regeneration and highlights the need for further transcriptome analysis across different tissues.

DATA DESCRIPTION

A. amurensis, a starfish species found in the northwestern Pacific, is known for its strong predatory behavior and impact on marine biodiversity. In this study, individuals were divided into a normal group and a 72-hour post-amputation group. De novo transcriptome assembly of the nerve cord identified 257,769 unigenes, which were functionally annotated using GO, KEGG, NR, and UniProt databases. Since only nerve cord tissue was analyzed, additional transcriptome studies on various tissues are required for a more comprehensive understanding of A. amurensis biology.

摘要

目的

本研究调查了多棘海盘车的神经索转录组,以探索其再生能力。通过比较正常组和截肢后72小时组之间的基因表达,确定了参与再生的关键基因。使用GO、KEGG、NR和UniProt数据库进行功能注释,深入了解了这些基因的生物学作用。本研究增进了对多棘海盘车再生的理解,并强调了对不同组织进行进一步转录组分析的必要性。

数据描述

多棘海盘车是一种在西北太平洋发现的海星物种,以其强烈的捕食行为和对海洋生物多样性的影响而闻名。在本研究中,个体被分为正常组和截肢后72小时组。对神经索进行从头转录组组装,鉴定出257,769个单基因,并使用GO、KEGG、NR和UniProt数据库对其进行功能注释。由于仅分析了神经索组织,因此需要对各种组织进行额外的转录组研究,以更全面地了解多棘海盘车的生物学特性。

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本文引用的文献

1
A Review of Asteroid Biology in the Context of Sea Star Wasting: Possible Causes and Consequences.海星消耗病背景下的小行星生物学综述:可能的原因和后果。
Biol Bull. 2022 Aug;243(1):50-75. doi: 10.1086/719928. Epub 2022 Jul 22.
2
Regeneration in Echinoderms: Molecular Advancements.棘皮动物的再生:分子进展
Front Cell Dev Biol. 2021 Dec 17;9:768641. doi: 10.3389/fcell.2021.768641. eCollection 2021.
3
Transcriptomic identification of starfish neuropeptide precursors yields new insights into neuropeptide evolution.转录组鉴定海星神经肽前体,为神经肽进化提供新见解。
Open Biol. 2016 Feb;6(2):150224. doi: 10.1098/rsob.150224.
4
Transcriptomic changes during regeneration of the central nervous system in an echinoderm.棘皮动物中枢神经系统再生过程中的转录组变化
BMC Genomics. 2014 May 12;15:357. doi: 10.1186/1471-2164-15-357.