Domino E F, Mathews B N, Tait S K
Biomed Mass Spectrom. 1979 Aug;6(8):331-4. doi: 10.1002/bms.1200060804.
The free urinary metabolites, homovanillic, indoleacetic and 5-hydroxyindoleacetic acid were measured in 24 h urines obtained from seven highly selected drug-free chronic schizophrenic patients and seven mentally normal control subjects on a low amine diet. Gas chromatography selected ion monitoring of silylated extracts was used to assay each metabolite using its own deuterated form as an internal standard. In the electron impact mode the positive ion fragments used to quantitate the amount of each metabolite in the urine were, respectively, m/z 209/211 for homovanillic acid, 319/321 for indoleacetic acid and 290/292 for 5-hydroxyindoleacetic acid. It was found essential that each compound be assayed using its own deuterated derivative as the internal standard. When expressed per mg creatinine, the homovanillic and 5-hydroxyindoleacetic acid levels of the chronic schizophrenic patients were in the normal range, but indoleacetic acid was slightly and statistically significantly higher, p = 0.01.
对7名经过严格挑选的无药物依赖的慢性精神分裂症患者和7名食用低胺饮食的精神正常对照者的24小时尿液中的游离尿代谢物高香草酸、吲哚乙酸和5-羟吲哚乙酸进行了测定。采用气相色谱选择离子监测硅烷化提取物，以各自的氘代形式作为内标物对每种代谢物进行测定。在电子轰击模式下，用于定量尿液中每种代谢物含量的正离子碎片分别为：高香草酸的m/z 209/211、吲哚乙酸的m/z 319/321和5-羟吲哚乙酸的m/z 290/292。发现必须使用各自的氘代衍生物作为内标物来测定每种化合物。以每毫克肌酐表示时，慢性精神分裂症患者的高香草酸和5-羟吲哚乙酸水平在正常范围内，但吲哚乙酸略高且具有统计学显著性差异，p = 0.01。
