白喉毒素片段的磷脂相互作用的pH依赖性
pH-dependence of the phospholipid interaction of diphtheria-toxin fragments.
作者信息
Montecucco C, Schiavo G, Tomasi M
出版信息
Biochem J. 1985 Oct 1;231(1):123-8. doi: 10.1042/bj2310123.
Abstract
Photoreactive phospholipids have been used to probe the lipid interaction of diphtheria toxin. Low pH values induce the membrane insertion of both the binding and enzymic fragments of the toxin. The efficiency of this process is much higher with asolectin than with egg lecithin (phosphatidylcholine)/cholesterol liposomes. The low-pH-induced interaction of the toxin fragments with the membrane hydrocarbon phase is more evident for the enzymic A-chain than for the binding B-chain, and it is fully reversed by returning the pH to neutrality.
摘要
光反应性磷脂已被用于探究白喉毒素的脂质相互作用。低pH值会诱导毒素的结合片段和酶片段插入膜中。该过程在大豆卵磷脂中比在卵磷脂（磷脂酰胆碱）/胆固醇脂质体中的效率要高得多。低pH诱导的毒素片段与膜烃相的相互作用对于酶活性A链比结合性B链更明显，并且通过将pH恢复到中性可完全逆转。
相似文献
1
pH-dependence of the phospholipid interaction of diphtheria-toxin fragments.白喉毒素片段的磷脂相互作用的pH依赖性
Biochem J. 1985 Oct 1;231(1):123-8. doi: 10.1042/bj2310123.
2
Binding of diphtheria toxin to phospholipids in liposomes.白喉毒素与脂质体中磷脂的结合。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1986-90. doi: 10.1073/pnas.77.4.1986.
3
Interactions of diphtheria toxin with lipid vesicles: determinants of ion channel formation.
Biochim Biophys Acta. 1987 Sep 18;903(1):48-55. doi: 10.1016/0005-2736(87)90154-4.
4
Aggregation and fusion of lipid vesicles induced by diphtheria toxin at low pH: possible involvement of the P site and the NAD+ binding site.
Biosci Rep. 1985 Mar;5(3):243-50. doi: 10.1007/BF01119594.
5
Lipid interaction of diphtheria toxin and mutants with altered fragment B. 1. Liposome aggregation and fusion.
Eur J Biochem. 1987 Dec 15;169(3):629-35. doi: 10.1111/j.1432-1033.1987.tb13654.x.
6
Lipid interaction of diphtheria toxin and mutants with altered fragment B. 2. Hydrophobic photolabelling and cell intoxication.
Eur J Biochem. 1987 Dec 15;169(3):637-44. doi: 10.1111/j.1432-1033.1987.tb13655.x.
7
Diphtheria toxin and its mutant crm 197 differ in their interaction with lipids.白喉毒素及其突变体crm 197与脂质的相互作用存在差异。
FEBS Lett. 1987 May 4;215(1):73-8. doi: 10.1016/0014-5793(87)80116-3.
8
Secondary structure changes of diphtheria toxin interacting with asolectin liposomes: an infrared spectroscopy study.
Biochimie. 1989 Jan;71(1):153-8. doi: 10.1016/0300-9084(89)90145-4.
9
pH-dependent bilayer destabilization and fusion of phospholipidic large unilamellar vesicles induced by diphtheria toxin and its fragments A and B.
Biochemistry. 1989 Apr 18;28(8):3406-13. doi: 10.1021/bi00434a040.
10
Lipid interaction of diphtheria toxin and mutants. A study with phospholipid and protein monolayers.
Eur J Biochem. 1991 Apr 23;197(2):481-6. doi: 10.1111/j.1432-1033.1991.tb15935.x.
引用本文的文献
1
Topography of the TH5 Segment in the Diphtheria Toxin T-Domain Channel.白喉毒素T结构域通道中TH5片段的拓扑结构。
J Membr Biol. 2016 Apr;249(1-2):181-96. doi: 10.1007/s00232-015-9859-9. Epub 2015 Dec 8.
2
Microsecond Simulations of the Diphtheria Toxin Translocation Domain in Association with Anionic Lipid Bilayers.白喉毒素转位结构域与阴离子脂质双层结合的微秒级模拟
J Phys Chem B. 2015 Sep 10;119(36):12074-85. doi: 10.1021/acs.jpcb.5b07909. Epub 2015 Aug 31.
3
Cellular internalisation of bacterial toxins.
Cytotechnology. 1991 Feb;5(Suppl 1):63-6. doi: 10.1007/BF00736811.
4
The membrane topography of the diphtheria toxin T domain linked to the a chain reveals a transient transmembrane hairpin and potential translocation mechanisms.与α链相连的白喉毒素T结构域的膜拓扑结构揭示了一种瞬时跨膜发夹结构和潜在的转运机制。
Biochemistry. 2009 Nov 3;48(43):10446-56. doi: 10.1021/bi9014665.
5
Induction of neutralizing antibodies against diphtheria toxin by priming with recombinant Mycobacterium bovis BCG expressing CRM(197), a mutant diphtheria toxin.用表达CRM(197)（一种突变的白喉毒素）的重组牛分枝杆菌卡介苗进行初次免疫，诱导产生抗白喉毒素的中和抗体。
Infect Immun. 2001 Feb;69(2):869-74. doi: 10.1128/IAI.69.2.869-874.2001.
6
A chimeric toxin to study the role of the 21 kDa GTP binding protein rho in the control of actin microfilament assembly.一种用于研究21 kDa GTP结合蛋白rho在肌动蛋白微丝组装调控中作用的嵌合毒素。
EMBO J. 1993 Mar;12(3):921-31. doi: 10.1002/j.1460-2075.1993.tb05733.x.
7
Refined structure of monomeric diphtheria toxin at 2.3 A resolution.分辨率为2.3埃的单体白喉毒素精细结构
Protein Sci. 1994 Sep;3(9):1464-75. doi: 10.1002/pro.5560030912.
8
Effect of pH on the interaction of botulinum neurotoxins A, B and E with liposomes.
Biochem J. 1989 Apr 1;259(1):47-53. doi: 10.1042/bj2590047.
9
On the membrane translocation of diphtheria toxin: at low pH the toxin induces ion channels on cells.关于白喉毒素的膜转位：在低pH值下，毒素可诱导细胞上形成离子通道。
EMBO J. 1988 Nov;7(11):3353-9. doi: 10.1002/j.1460-2075.1988.tb03207.x.
10
Dynamic properties of membrane proteins: reversible insertion into membrane vesicles of a colicin E1 channel-forming peptide.膜蛋白的动态特性：大肠杆菌素E1通道形成肽可逆插入膜泡
Proc Natl Acad Sci U S A. 1988 Oct;85(20):7531-5. doi: 10.1073/pnas.85.20.7531.
本文引用的文献
1
ACTION SPECTRUM FOR THE APPEARANCE OF THE 520 MILLIMICRON DIFFERENCE BAND IN ILLUMINATED CHLORELLA CELLS.光照下小球藻细胞中520毫微米差异带出现的作用光谱。
Biophys J. 1964 Mar;4(2):107-13. doi: 10.1016/s0006-3495(64)86772-2.
2
The entry of diphtheria toxin into the mammalian cell cytoplasm: evidence for lysosomal involvement.白喉毒素进入哺乳动物细胞质：溶酶体参与的证据。
J Cell Biol. 1980 Dec;87(3 Pt 1):849-54. doi: 10.1083/jcb.87.3.849.
3
Diphtheria toxin entry into cells is facilitated by low pH.低pH值有助于白喉毒素进入细胞。
J Cell Biol. 1980 Dec;87(3 Pt 1):828-32. doi: 10.1083/jcb.87.3.828.
4
Lipid insertion of cholera toxin after binding to GM1-containing liposomes.霍乱毒素与含GM1的脂质体结合后进行脂质插入。
J Biol Chem. 1981 Nov 10;256(21):11177-81.
5
Micellar gangliosides mediate the lipid insertion of cholera toxin protomer A.
Biochim Biophys Acta. 1982 Nov 22;692(3):339-44. doi: 10.1016/0005-2736(82)90382-0.
6
The transmembrane domain of glycophorin A as studied by cross-linking using photoactivatable phospholipids.使用光活化磷脂交联法研究血型糖蛋白A的跨膜结构域。
J Biol Chem. 1982 Apr 25;257(8):4152-61.
7
Binding of diphtheria toxin to phospholipids in liposomes.白喉毒素与脂质体中磷脂的结合。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1986-90. doi: 10.1073/pnas.77.4.1986.
8
Hydrophobic labeling of a single leaflet of the human erythrocyte membrane.人红细胞膜单小叶的疏水标记
Biochemistry. 1983 Aug 2;22(16):3812-20. doi: 10.1021/bi00285a016.
9
Evidence for direct insertion of fragments A and B of diphtheria toxin into model membranes.白喉毒素A片段和B片段直接插入模型膜的证据。
J Biol Chem. 1984 Oct 10;259(19):12226-33.
10
Diphtheria toxin induces fusion of small unilamellar vesicles at low pH.
Biochim Biophys Acta. 1984 Aug 8;775(1):31-6. doi: 10.1016/0005-2736(84)90231-1.
Zotero 插件