Montecucco C, Schiavo G, Tomasi M
Biochem J. 1985 Oct 1;231(1):123-8. doi: 10.1042/bj2310123.
Photoreactive phospholipids have been used to probe the lipid interaction of diphtheria toxin. Low pH values induce the membrane insertion of both the binding and enzymic fragments of the toxin. The efficiency of this process is much higher with asolectin than with egg lecithin (phosphatidylcholine)/cholesterol liposomes. The low-pH-induced interaction of the toxin fragments with the membrane hydrocarbon phase is more evident for the enzymic A-chain than for the binding B-chain, and it is fully reversed by returning the pH to neutrality.
光反应性磷脂已被用于探究白喉毒素的脂质相互作用。低pH值会诱导毒素的结合片段和酶片段插入膜中。该过程在大豆卵磷脂中比在卵磷脂（磷脂酰胆碱）/胆固醇脂质体中的效率要高得多。低pH诱导的毒素片段与膜烃相的相互作用对于酶活性A链比结合性B链更明显，并且通过将pH恢复到中性可完全逆转。
