Lee T D, Legesse K, Hawke D H, Shively J E, Rothman B S, Mayeri E
Biochem Biophys Res Commun. 1985 Oct 30;132(2):520-5. doi: 10.1016/0006-291x(85)91164-7.
Three peptides isolated from the atrial glands of Aplysia californica were analysed by Fast Atom Bombardment Mass Spectrometry. Survey scans over the mass range 1650 to 7500 at 500 resolution were used to locate signals for the protonated molecular ion and two subunits which result from cleavage of a single disulfide bond. A more accurate mass determination was made by accumulating scans over a narrow mass range. The amounts of sample used for each measurement ranged between 10 and 30 pmoles. Measured mass values are within 0.5 amu of calculated average molecular weights. Results illustrate the utility of the technique for accurate molecular weight determinations on limited quantities of high molecular weight peptides.
利用快原子轰击质谱法对从加州海兔心房腺中分离出的三种肽进行了分析。采用分辨率为500的扫描模式，在1650至7500的质量范围内进行全扫描，以定位质子化分子离子和因单个二硫键断裂产生的两个亚基的信号。通过在狭窄质量范围内累积扫描进行更精确的质量测定。每次测量所用样品量在10至30皮摩尔之间。测量的质量值与计算出的平均分子量相差在0.5原子质量单位以内。结果表明该技术在对有限量的高分子量肽进行精确分子量测定方面的实用性。
