Williams D H, Bradley C V, Santikarn S, Bojesen G
Biochem J. 1982 Jan 1;201(1):105-17. doi: 10.1042/bj2010105.
A detailed study of the mass spectra of peptides produced by the new technique of fast-atom bombardment is reported. Molecular weights of unmodified peptides containing up to 21 amino acids have been determined. In favourable cases, the molecular-weight determination may be made on as little as 0.1 nmol of sample. Positive-ion and negative-ion spectra are obtained with equal facility. With sample sizes in the range 2-50nmol, sequence information can be obtained in many cases. The technique represents an important contribution to structural studies on peptides, since (i) blocked peptides may be studied, (ii) no prior formation of chemical derivatives is necessary (except for distinction between lysine and glutamine), (iii) sensitivity is good, (iv) large peptides, and, in some cases, mixtures of peptides, can be studied, and (v) the technique is easy to operate, with ions being produced over a long period (5-30 min).
本文报道了对快速原子轰击新技术产生的肽的质谱进行的详细研究。已测定了含有多达21个氨基酸的未修饰肽的分子量。在有利的情况下，仅用0.1纳摩尔的样品就可以进行分子量测定。正离子和负离子光谱的获取同样容易。对于2 - 50纳摩尔范围内的样品量，在许多情况下都可以获得序列信息。该技术对肽的结构研究做出了重要贡献，因为：（i）可以研究封闭的肽；（ii）无需事先形成化学衍生物（赖氨酸和谷氨酰胺的区分除外）；（iii）灵敏度良好；（iv）可以研究大肽，在某些情况下还可以研究肽混合物；（v）该技术易于操作，离子可在较长时间（5 - 30分钟）内产生。
